Strain Detail Sheet

Strain Name:
C.Cg-Ighb Igh-Jtm1Khyk/KhykMmnc
Stock Number:
068105-UNC
Citation ID:
RRID:MMRRC_068105-UNC
Other Names:
Vh11KI

Strain Information

Gene Details

Igh-Jtm1Khyk
Name: immunoglobulin heavy chain, joining region; targeted mutation 1, Kyoko Hayakawa
Synonyms: Vh11+ NeoR-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knock-In
Igh-J
Name: immunoglobulin heavy chain, joining region
Synonyms: Jh
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knock-In
Igh
Name: immunoglobulin heavy chain complex
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Ighb
Name:
Type: Other Genome Feature
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Genetic Alterations

A targeting vector was designed to insert a VH11DHJH1 gene segment into the locus, with a floxed neo. The neo was subsequently removed via cre-mediated recombination.

Curation note: The data provided for this strain are incomplete and cannot be uniquely tied to genetic entities. The identity of the Ighb allele, for example, is uncertain; it may be a synonym for Ighg2ab (MGI:6515767). Please refer to the cited publications form more information.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    RW-4 derived from 129X1/SvJ

    Strain Description

    Phenotype
    Increase in anti-PC B cells expressing endogenous Vk9id light chain combined with the transgenic Vh11u heavy chain.
    MeSH Terms
    • Adoptive Transfer
    • Animals
    • Animals, Newborn
    • B-Lymphocyte Subsets/cytology
    • B-Lymphocyte Subsets/immunology
    • B-Lymphocyte Subsets/metabolism
    • B-Lymphocyte Subsets/transplantation
    • Cell Aggregation/genetics
    • Cell Aggregation/immunology
    • Cell Communication/immunology
    • Cell Differentiation/genetics
    • Cell Differentiation/immunology
    • Dendritic Cells, Follicular/cytology
    • Dendritic Cells, Follicular/immunology
    • Female
    • Immunoglobulin D/biosynthesis
    • Immunoglobulin Heavy Chains/biosynthesis
    • Immunoglobulin Heavy Chains/genetics
    • Immunoglobulin Idiotypes/biosynthesis
    • Immunoglobulin Idiotypes/genetics
    • Immunoglobulin Variable Region/biosynthesis
    • Immunoglobulin Variable Region/genetics
    • Immunoglobulin kappa-Chains/biosynthesis
    • Immunoglobulin kappa-Chains/genetics
    • Lymphocyte Count
    • Male
    • Mice
    • Mice, Inbred BALB C
    • Mice, Knockout
    • Mice, Transgenic
    • Phosphatidylcholines/immunology
    • Receptors, CXCR5
    • Receptors, Chemokine
    • Receptors, Cytokine/biosynthesis
    • Spleen/cytology
    • Spleen/immunology
    Strain Development
    Gene targeting was performed by inserting a Vh11DhJh1 gene segment cloned from a Vh11/Vk9 anti-PtC hydridoma (2-2G4) into a Jh locus knock-in construct that contains the neomycin resistance gene flanked by loxP sites, then transfecting the ES line RW4 (derived from 129X1/SvJ). Chimeric male mice were bred, and upon obtaining germline transmission, Vh11+ mice were crossed with Cre deleter mice to eliminate the neo gene. Vh11+NeoR- offspring were then crosssed to CB17 mice for 8 generations.
    Suggested Control Mice

    CB17 (https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611)

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Kyoko Hayakawa, M.D., Fox Chase Cancer Center.
    Primary Reference

    Wen L, Shinton SA, Hardy RR, Hayakawa K. Association of B-1 B cells with follicular dendritic cells in spleen. J Immunol. 2005 Jun 1;174(11):6918-26. doi: 10.4049/jimmunol.174.11.6918. (Medline PMID: 15905534)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    White
    Eye
    Pink

    Breeding

    MMRRC Breeding System
    Backcrossing
    Generation

    N8 (CB17 - see https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611 and additional information from MGI: http://www.informatics.jax.org/inbred_strains/mouse/docs/C.shtml)

    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.