Strain Name:
B6J.Cg-Foxl1tm2(cre/ERT2)Khk/Mmjax
Stock Number:
068163-JAX
Citation ID:
RRID:MMRRC_068163-JAX
Other Names:
FoxL1CreER-tdTomato

Strain Information

Foxl1tm2(cre/ERT2)Khk
Name: forkhead box L1; targeted mutation 1, Klaus Kaestner
Type: Allele
Species: Multi-species
Chromosome: 8
Foxl1
Name: forkhead box L1
Synonyms: fkh-6, Fkh6, fkh6, FREAC7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14241
HGNC: HGNC:3817
Homologene: 48335
Genetic Alterations
Targeted insertion of a tdTomato fluorescent reporter and tamoxifen-dependent Cre recombinase into the FoxL1 locus directly after the endogenous Foxl1 translational start site.
ES Cell Line
v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
Phenotype
The FoxL1CreER-tdTomato knock-in allele is a FoxL1 null allele. Heterozygotes are phenotypically normal, while homozygotes display developmental delays in gut formation and abnormal proliferation and architecture of the gastrointestinal epithelium.

Note: This model should not be used as homozygotes unless an analysis of the FoxL1 null phenotype is intended.
Strain Development
FoxL1CreERT2-2A-tdTomato mice were derived by inserting a CreERT2-2A-tdTomato-PGK-NeomycinR cassette into the endogenous FoxL1 locus via homology-directed repair in mouse V6.5 embryonic stem cells (B6129S4F1 male). The targeting vector was generated by In-Fusion cloning, and arms of homology were amplified from genomic DNA of the V6.5 cells to be targeted; all components were verified by Sanger sequencing. Two hundred base pairs of the Foxl1 coding sequence downstream of the ATG were deleted rendering this reporter allele null for FoxL1 protein expression. The linearized targeting vector was introduced by nucleofection into the ESCs after which the cultures were subjected to G418 selection for 7 days and drug-resistant colonies were subcloned and screened for proper insertion. Insertion was validated at both the 5’ and 3’ insertion sites. Several correctly targeted clones were identified, and one was injected into C57BL/6 blastocysts resulting in the generation of several high-contribution male chimeras. Chimeras were backcrossed six times to C57BL/6J females to establish the FoxL1CreERT2-2A-tdTomato strain. FoxL1CreERT2-2A-tdTomato mice were subsequently routinely genotyped using a PCR reaction enabling resolution of both wild-type and FoxL1CreERT2-2A-tdTomato alleles.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
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Donor
Klaus Kaestner, Ph.D., University of Pennsylvania.
Primary Reference
In preparation, submitted, or in press

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N6 (C57BL/6J)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068163-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
068163-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.