Strain Detail Sheet

Strain Name    :

B6;129P2-Lrp6Gt(Ex187)Byg/Mmucd

Stock Number :

030661-UCD

Other Names   :

LRP6, B6.129P2-Lrp6Gt(Ex187)Byg/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Lrp6Gt(Ex187)Byg
Name: gene trap Ex187, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Lrp6
Name: low density lipoprotein receptor-related protein 6
Chromosome: 6
Alteration at locus: Gene Trap

Genetic Alterations:

Genotype Determination:

ES Cell Line: CGR8 derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Perinatally lethal, Skeletal defects, Limb defects, microophthalmia, Urogenital defects, Neural defects

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Lrp6Gt(Ex187)Byg/Lrp6+
        involves: 129/Sv * 129P2/OlaHsd * C57BL/6J * FVB/N
  • skeleton phenotype
    • decreased bone mineral density (MGI Ref ID J:111359)
      • significantly reduced vs wild-type; present in all regions examined so values are not disproportionately reduced in animals with limb defects
Lrp6Gt(Ex187)Byg/Lrp6Gt(Ex187)Byg
        involves: 129P2/OlaHsd
  • embryogenesis phenotype
    • incomplete somite formation (MGI Ref ID J:93008)
      • axial truncation occurs in the lumbar region
  • reproductive system phenotype
    • abnormal external female genitalia morphology (MGI Ref ID J:155250)
      • complete absence
    • abnormal external male genitalia morphology (MGI Ref ID J:155250)
      • complete absence
Lrp6Gt(Ex187)Byg/Lrp6Gt(Ex187)Byg
        involves: 129P2/OlaHsd * C57BL/6
  • mortality/aging
    • complete perinatal lethality (MGI Ref ID J:64989)
      • homozygotes die at birth
  • embryogenesis phenotype
    • abnormal apical ectoderm al ridge (MGI Ref ID J:64989)
      • homozygotes fail to maintain the apical ectodermal ridge
    • abnormal paraxial mesoderm (MGI Ref ID J:64989)
      • loss of paraxial mesoderm is seen at E9.5
    • incomplete somite formation (MGI Ref ID J:64989)
      • caudal somites are absent at E10.5
    • sirenomelia (MGI Ref ID J:64989)
    • spina bifida (MGI Ref ID J:64989)
      • about half of homozygotes display spina bifida and/or exencephaly
  • nervous system phenotype
    • abnormal cerebellum morphology (MGI Ref ID J:64989)
      • at birth the cerebellum is disorganized
    • abnormal midbrain morphology (MGI Ref ID J:64989)
      • at E14.5 the caudal midbrain is absent
      • absent inferior colliculus (MGI Ref ID J:64989)
        • at birth the inferior colliculus is absent
    • abnormal midbrain-hindbrain boundary development (MGI Ref ID J:64989)
      • at E10.5 the midbrain hindbrain boundary is less distinct
    • exencephaly (MGI Ref ID J:64989)
      • about half of homozygotes display spina bifida and/or exencephaly
    • spina bifida (MGI Ref ID J:64989)
      • about half of homozygotes display spina bifida and/or exencephaly
  • skeleton phenotype
    • absent vertebrae (MGI Ref ID J:64989)
      • vertebrae caudal to the lumbar region are absent
  • limbs/digits/tail phenotype
    • abnormal apical ectodermal ridge (MGI Ref ID J:64989)
      • homozygotes fail to maintain the apical ectodermal ridge
    • abnormal digit morphology (MGI Ref ID J:64989)
      • some of the remaining forelimb digits have ectopic tendons
      • oligodactyly (MGI Ref ID J:64989)
        • one or more posterior digits are missing on the forelimb and variable loss of anterior digits is seen
    • abnormal tail bud morphology (MGI Ref ID J:64989)
      • at E8.5, the tail bud is reduced in size and at E9.5 sections through the tail bud reveal excess neural tissue and loss of paraxial mesoderm
    • sirenomelia (MGI Ref ID J:64989)

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural

Strain Origin

Donor: William C. Skarnes, Ph.D., Wellcome Trust Sanger Institute.

Primary Reference:

Pinson KI; Brennan J; Monkley S; Avery BJ; Skarnes WC, An LDL-receptor-related protein mediates Wnt signalling in mice, Nature 2000 Sep 28;407(6803):535-8 (Medline PMID: 11029008)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System: Backcross followed by sib-mating

Breeding Scheme(s):

  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate

Generation: N4

Overall Breeding Performance:

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Undetermined Undetermined

Average litter size: Undetermined

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030661-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030661-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030661-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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