Strain Detail Sheet

Strain Name:
Soriano ES Feeder cell line SNL 76/7 STO
Stock Number:
015892-UCD
Citation ID:
RRID:MMRRC_015892-UCD
Major Collection:
Soriano Gene Trap Lines

Gene Information

Gene Details

Genetic Alterations
Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.
ES Cell Line
AK7.1 (MMRRC Stock Number 015891-UCD)

Related Protocols

Strain Origin

Donor
Soriano, Philippe, Mount Sinai Medical Center, NY (See Soriano Lab Overview)
Primary Reference

Friedrich G, Soriano P., Insertional mutagenesis by retroviruses and promoter traps in embryonic stem cells. Methods Enzymol 1993;225:681-701.(Medline PMID: 8231879 )

Chen WV, Soriano P. Gene trap mutagenesis in embryonic stem cells. Methods Enzymol. 2003;365:367-86. (Medline PMID: 14696359)

Colony and Husbandry Information

Special Considerations

SNL 76/7 STO feeder cells express leukemia inhibitory factor (LIF) and neomycin phosphotransferase (Neo).

Soriano Parental Cell Line AK7

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
015892-UCD-CTRL Soriano Gene Trap Lines High Passage Parental cell line Soriano ES Feeder cell line SNL 76/7 STO (suitable for DNA extraction, control purposes; not for germline transmission) $148.67 / Non-Profit Vial Suitable for DNA extraction, control purposes but not for germline transmission.
015892-UCD-CELL Soriano Gene Trap Lines Low Passage Parental cell line Soriano ES Feeder cell line SNL 76/7 STO (suitable for electroporation and germline transmission) $740.70 / Non-Profit Vial Suitable for a single electroporation and germline transmission.
015892-UCD-ULTRA Soriano Gene Trap Lines Ultra-low Passage Parental cell line Soriano ES Feeder cell line SNL 76/7 STO (suitable for multiple electroporations and germline transmission) $2,059.70 / Non-Profit Vial Suitable for a multiple electroporation and germline transmission.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.