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Strain Detail Sheet

Published: 06/16/2019
Revised: 11/07/2025

Strain Name:
B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
Stock Number:
034848-JAX
Citation ID:
RRID:MMRRC_034848-JAX
Other Names:
This strain was formerly available as JAX Stock #008730. 5XFAD line Tg6799, FXFAD APP/PS1

Strain Information

Gene Details

Psen1
Name: presenilin 1
Synonyms: S182, PS1, PS-1, presenilin-1, Ad3h
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19164
VEGA: 12
HGNC: HGNC:9508
Homologene: 7186

Additional Resources
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ClinGen
Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas
Name: transgene insertion 6799, Robert Vassar
Synonyms: Tg(APP*Swe*Fl*Lon,PSEN1*M146L*L286V)6799Vas, 5XFAD APP/PS1, Tg-5xFAD, 5XFAD, 5XFAD line Tg6799, Tg6799
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
These "5XFAD" transgenic mice overexpress both mutant human APP(695) with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) Familial Alzheimer's Disease (FAD) mutations and human PS1 harboring two FAD mutations, M146L and L286V.
Genotype Determination

Strain Description

Phenotype
Homozygote: Phenotype is expected to be similar to hemizygote phenotype.
Hemizygous: Mice are viable and fertile. expression of both transgenes is regulated by neural-specific elements of the mouse Thy1 promoter to drive overexpression in the brain. Mice from this founder line have high APP expression correlating with high burden and accelerated accumulation of the 42 amino acid species of beta-amyloid (Abeta-42). 5XFAD mice generate Abeta-42 almost exclusively and rapidly accumulate massive cerebral levels. On the B6SJL F1 genetic background (MMRRC:034840), intraneuronal Abeta-42 accumulation is observed starting at 1.5 months of age, just prior to amyloid deposition and gliosis, which begins at two months of age. On a congenic C57BL/6J genetic background (this strain) it has been the observation of the MMRRC that this phenotype is not as robust as that demonstrated in the B6SJL hybrid background (view data). In addition, these mice have reduced synaptic marker protein levels, increased p25 levels, neuron loss, and memory impairment in the Y-maze test. 5XFAD transgenic mice rapidly recapitulate major features of Alzheimer's Disease amyloid pathology and may be useful models of intraneuronal Abeta-42 induced neurodegeneration and amyloid plaque formation.

This strain does not carry the retinal degeneration allele Pde6brd1.

Parental origin of the 5xFAD transgene can affect plaque deposition:
Sasmita et al., 2025 Neuron [PMID:39837326] describes breeding C57BL/6 5xFAD hemizygous mice to wildtype (noncarrier) mice that they found paternal inheritance of the transgene (n=20 hemizygous offspring) led to a 2-fold higher plaque burden compared with maternal inheritance (n=11 hemizygous offspring) via quantitative light-sheet microscopy. Importantly, the extent of the observed effect is comparable with the known sex dimorphism of the 5xFAD model. This effect was not due to gestation-in or rearing-by 5xFAD females. Immunoblotting suggested that transgenic inheritance modulates transgenic protein expression, potentially due to genomic imprinting of a CpG island within the Thy1.2 promoter. Taken together, these findings underscore the importance of the AD research community to [1] systematically report breeding schemes in publications, [2] ensure the comparison of inheritance-matched cohorts, and [3] critically review past publications for inheritance mismatches as a confounding variable.
MeSH Terms
  • Alzheimer Disease/genetics
  • Alzheimer Disease/pathology
  • Amyloid beta-Peptides/genetics
  • Animals
  • Cell Count
  • Memory Disorders/genetics
  • Memory Disorders/pathology
  • Mice
  • Mice, Transgenic
  • Mutation
  • Nerve Degeneration/genetics
  • Nerve Degeneration/pathology
  • Neurofibrillary Tangles/genetics
  • Neurofibrillary Tangles/pathology
  • Neurons/pathology
  • Plaque, Amyloid/genetics
  • Plaque, Amyloid/pathology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Robert Vassar, Ph.D., Northwestern University
Primary Reference
Oakley H, Cole SL, Logan S, Maus E, Shao P, Craft J, Guillozet-Bongaarts A, Ohno M, Disterhoft J, Van Eldik L, Berry R, Vassar R. Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation. J Neurosci. 2006 Oct 4;26(40):10129-40 (Medline PMID: 17021169)
Additional References

Sasmita AO, Ong EC, Nazarenko T, Mao S, Komarek L, Thalmann M, Hantakova V, Spieth L, Berghoff SA, Barr HJ, Hingerl M, Börensen F, Hirrlinger J, Simons M, Stevens B, Depp C, Nave KA. Parental origin of transgene modulates amyloid-β plaque burden in the 5xFAD mouse model of Alzheimer's disease. Neuron. 2025 Mar 19;113(6):838-846.e4. doi: 10.1016/j.neuron.2024.12.025. Epub 2025 Jan 20. (Medline PMID: 39837326)

Strain Development
A transgene was designed with a mutant human amyloid beta (A4) precursor protein (APP) cDNA sequence (altered to include the APP K670N/M671L (Swedish) + I716V (Florida) + V717I (London) Familial Alzheimer's Disease (FAD) mutations) inserted into exon 2 of the mouse Thy1 gene. A second transgene was designed with a mutant human presenilin 1 (Alzheimer disease 3) (PSEN1 or PS1) cDNA sequence (altered to include the PS1 M146L + L286V FAD mutations) inserted into exon 2 of the mouse Thy1 gene. Both transgenes were added together in equal proportions and co-injected into the pronuclei of single-cell "C57/B6XSJL" hybrid embryos. Founders from the highest APP expressing line (Tg6799) were bred with (B6/SJL)F1 for more than 10 generations with stable germ-line transmission and expression of both transgenes, demonstrating that these "5XFAD" mice breed as single transgenics. The mice were then backcrossed to C57BL/6J mice using a speed congenic protocol and the retinal degeneration allele Pde6brd1 was bred out of the strain. Of note, the APP transgene includes the 5' untranslated region and thus contains a putative interleukin-1beta translational enhancer element.

Parental origin of the 5xFAD transgene can affect plaque deposition:
Sasmita et al., 2025 Neuron [PMID:39837326] describes breeding C57BL/6 5xFAD hemizygous mice to wildtype (noncarrier) mice that they found paternal inheritance of the transgene (n=20 hemizygous offspring) led to a 2-fold higher plaque burden compared with maternal inheritance (n=11 hemizygous offspring) via quantitative light-sheet microscopy. Importantly, the extent of the observed effect is comparable with the known sex dimorphism of the 5xFAD model. This effect was not due to gestation-in or rearing-by 5xFAD females. Immunoblotting suggested that transgenic inheritance modulates transgenic protein expression, potentially due to genomic imprinting of a CpG island within the Thy1.2 promoter. Taken together, these findings underscore the importance of the AD research community to [1] systematically report breeding schemes in publications, [2] ensure the comparison of inheritance-matched cohorts, and [3] critically review past publications for inheritance mismatches as a confounding variable.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

When maintaining a live colony, hemizygous mice may be bred to C57BL/6J.

This strain ships with remnant UID MiniMax microchips implanted subcutaneously in the back. For more information on these RFID chips, including our FAQ, please visit our Mouse Identification page.

Breeding Considerations
  • In 2019-2020, The Jackson Laboratory public distribution colony was maintained via breeding hemizygous mice with noncarrier (wildtype) mice from the colony [an approximately equal mix of HEMI females x Noncarrier male units and Noncarrier females x HEMI male units].
  • In 2021, our public distribution colony maintenance plan was transitioned to breeding hemizygous mice with C57BL/6J [an approximately equal mix of HEMI females x C57BL/6J male units and C57BL/6J females x HEMI male units]. This was similar as of February 2025.
  • Following the March 2025 publication finding that parental origin of the 5xFAD transgene can affect plaque deposition (see Strain Development section above), our Stock No. 008730 public distribution colony maintenance plan was transitioned to breeding C57BL/6J females with hemizygous males. As of June 2025, ~75% of our breeding units are C57BL/6J females x hemizygous male.

Of note, researchers wishing to obtain offspring specifically generated via hemizygous females x C57BL/6J male breeding unit can do so via ordering JAX Stock No. 032883 mice.

Health Status Report

Colony's Current Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Order Information

Availability Level

Colony sized to accommodate the delivery of study cohorts. Please contact the distributing center directly for more details at csmmrrc@jax.org.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
034848-JAX-HET-F
034848-JAX-HET-M
034848-JAX-WT-F
034848-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
 $147.00 / $147.00
Non-Profit / For-Profit		 Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
034848-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.