Strain Name:
C57BL/6J-MtgxR6412Btlr/Mmmh
Stock Number:
044385-MU
Citation ID:
RRID:MMRRC_044385-MU
Other Names:
R6412 (G1)
Major Collection:

Strain Information

Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: Ick, 2210420N10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Xdh
Name: xanthine dehydrogenase
Synonyms: XO, Xox1, Xor, Xox-1, xanthine oxidase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22436
VEGA: 17
Homologene: 324
Rag1
Name: recombination activating 1
Synonyms: Rag-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19373
HGNC: HGNC:9831
Homologene: 387
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Rsrc1
Name: arginine/serine-rich coiled-coil 1
Synonyms: 1200013F24Rik, SRrp53
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66880
Homologene: 41147
BC025920
Name: cDNA sequence BC025920
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268319
Lct
Name: lactase
Synonyms: LOC226413, LPH, Lphl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Skic2
Name: SKI2 subunit of superkiller complex
Synonyms: Ski2w, 4930534J06Rik, Skiv2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 108077
Homologene: 123971
Ret
Name: ret proto-oncogene
Synonyms: c-Ret, RET51, RET9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19713
HGNC: HGNC:9967
Homologene: 7517
Spocd1
Name: SPOC domain containing 1
Synonyms: OTTMUSG00000009522
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 622480
Homologene: 83439
Zfp990
Name: zinc finger protein 990
Synonyms: Gm13225
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 101056073
Itgb2l
Name: integrin beta 2-like
Synonyms: pactolus, 5033406G21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16415
HGNC: HGNC:6155
Fbxw26
Name: F-box and WD-40 domain protein 26
Synonyms: Gm5163
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382109
Homologene: 110776
Vwf
Name: Von Willebrand factor
Synonyms: B130011O06Rik, 6820430P06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22371
Homologene: 466
Thbs2
Name: thrombospondin 2
Synonyms: Thrombospondin-2, Thbs-2, TSP2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21826
VEGA: 17
Homologene: 2438
Actl11
Name: actin-like 11
Synonyms: 4921517D21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67722
Homologene: 69412
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384534
Homologene: 129605
Luzp2
Name: leucine zipper protein 2
Synonyms: 9330154K17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233271
Homologene: 45618
Chst8
Name: carbohydrate sulfotransferase 8
Synonyms: 1500011J21Rik, GalNAc4ST-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68947
Homologene: 41483
Kbtbd12
Name: kelch repeat and BTB (POZ) domain containing 12
Synonyms: 4833415F11Rik, 4933428M03Rik, Klhdc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74589
Homologene: 52613
Or4b1b
Name: olfactory receptor family 4 subfamily B member 1B
Synonyms: Olfr1272, GA_x6K02T2Q125-51636504-51635578, MOR227-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258982
HGNC: HGNC:8290
Homologene: 133649
Wdr81
Name: WD repeat domain 81
Synonyms: MGC32441, shakey 5, nur5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192652
Homologene: 13983
Vmn1r223
Name: vomeronasal 1 receptor 223
Synonyms: Gm11330
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100036518
Homologene: 110880
Or5l14
Name: olfactory receptor family 5 subfamily L member 14
Synonyms: GA_x6K02T2Q125-49446395-49445457, Olfr1157, MOR174-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258846
Homologene: 17402
Bhlhe23
Name: basic helix-loop-helix family, member e23
Synonyms: beta-cell E-box transactivating factor 4, Bhlhb4, A930001L02Rik, BETA4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 140489
Homologene: 50956
Gm3264
Name: predicted gene 3264
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100041306
Homologene: 115686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 128,327,718 bp
  • A to T, chromosome 2 at 87,962,349 bp
  • G to T, chromosome 2 at 90,281,858 bp
  • A to G, chromosome 2 at 101,642,520 bp
  • A to G, chromosome 2 at 180,776,170 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • C to T, chromosome 4 at 129,953,572 bp
  • C to A, chromosome 4 at 145,537,568 bp
  • T to C, chromosome 5 at 124,392,714 bp
  • C to T, chromosome 6 at 18,285,604 bp
  • T to C, chromosome 6 at 88,618,656 bp
  • C to T, chromosome 6 at 118,184,284 bp
  • T to C, chromosome 6 at 125,679,316 bp
  • A to G, chromosome 7 at 10,171,009 bp
  • T to C, chromosome 7 at 34,676,079 bp
  • T to C, chromosome 7 at 55,058,046 bp
  • T to C, chromosome 9 at 48,946,519 bp
  • T to C, chromosome 9 at 78,139,976 bp
  • A to G, chromosome 9 at 107,929,917 bp
  • T to A, chromosome 9 at 109,732,647 bp
  • T to C, chromosome 10 at 81,609,361 bp
  • G to T, chromosome 11 at 75,451,163 bp
  • G to T, chromosome 13 at 23,249,655 bp
  • A to G, chromosome 14 at 4,871,199 bp
  • T to C, chromosome 16 at 96,427,729 bp
  • A to C, chromosome 17 at 14,677,077 bp
  • A to G, chromosome 17 at 34,840,300 bp
  • T to C, chromosome 17 at 73,935,907 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6412 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044385-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.