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Availability & Fees Order this Strain
Mice lacking both members of the 4930567H17Rik gene family (4930567H17Rik and Gm14725) do not have detectable defects in male fertility, fecundity, spermatogenesis, or in gene regulation, but do show differences in sperm head morphology, suggesting a potential role in sperm function.
Mice lacking the X-linked palindrome-associated 4930567H17Rik gene family were generated using a CRISPR Cas9 strategy. To generate mice lacking the 4930567H17Rik gene family (4930567H17Rik∆CDS/Y ), C57BL/6 J X SJL hybrid females were crossed with existing 4930567H17Rik+/Y mice. Zygotes were injected with Cas9 protein, a single-stranded oligonucleotide donor, and dual sgRNAs targeting each 4930567H17Rik gene copy to achieve a ~ 650 base pair deletion within each copy on both palindrome arms. An F1 male carrying a deletion of both 4930567H17Rik coding sequences in cis (4930567H17Rik∆CDS/Y ) was bred to a C57BL/6 J female to generate 4930567H17Rik∆CDS/+ female mice. 4930567H17Rik∆CDS/+ females were backcrossed to C57BL/6 J males to generate 4930567H17Rik∆CDS/Y mice. Mice were further backcrossed at least 10 generations.
Stark-Dykema ER, Dulka EA, Gerlinger ER, Mueller JL. X-linked palindromic gene families 4930567H17Rik and Mageb5 are dispensable for male mouse fertility. Sci Rep. 2022 May 20;12(1):8554. doi: 10.1038/s41598-022-12433-9. (Medline PMID: 35595785)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Shaina Vera.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Shaina Vera
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.