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Math5HA
These mice exhibit a wild-type phenotype. Expression of the HA-tagged ATOH protein allows researchers to use immunological detection methods to further studies of retinal ganglion cell development. The HA allele has been used for chromatin immunoprecipitation, immunostaining and triplex competitive RT-PCR experiments to identify downstream targets, chart protein expression, and assess enhancer deletion effects, respectively.
A targeting vector was designed to insert sequences encoding three copies the hemagglutinin epitope YPYDVPDYA (HA-tag) from the influenza virus A, and a Neo cassette flanked by two loxP sites on the 3’ end of the Atoh coding sequence. The construct was transfected into G4 (129xC57BL/6 F1 ) embryonic stem (ES) cells. Correctly targeted ES cells were injected into albino C57BL/6 blastocysts. Offspring were mated to C57BL/6 mice. At some later time the floxed neo was removed by mating with a CMV-cre mouse line. The mice were then expanded by serial back-cross to C57BL/6J (JAX000664, >N10). Since then, homozygotes have been maintained by filial intercross (>F8).
Fu X, Kiyama T, Li R, Russell M, Klein WH, Mu X. Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse. Dev Dyn. 2009 Sep;238(9):2309-17. doi: 10.1002/dvdy.21974. (Medline PMCID: 3401478)
Mao CA, Cho JH, Wang J, Gao Z, Pan P, Tsai WW, Frishman LJ, Klein WH. Reprogramming amacrine and photoreceptor progenitors into retinal ganglion cells by replacing Neurod1 with Atoh7. Development. 2013 Feb 1;140(3):541-51. doi: 10.1242/dev.085886. (Medline PMCID: 3561791)
Miesfeld JB, Glaser T, Brown NL. The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody. Gene Expr Patterns. 2018 Jan;27:114-121. doi: 10.1016/j.gep.2017.11.006. Epub 2017 Dec 7. (Medline PMCID: 5835195)
Miesfeld JB, Ghiasvand NM, Marsh-Armstrong B, Marsh-Armstrong N, Miller EB, Zhang P, Manna SK, Zawadzki RJ, Brown NL, Glaser T. The Atoh7 remote enhancer provides transcriptional robustness during retinal ganglion cell development. Proc Natl Acad Sci U S A. 2020 Sep 1;117(35):21690-21700. doi: 10.1073/pnas.2006888117. Epub 2020 Aug 17. (Medline PMCID: 7474671)
Ge Y, Wu F, Cheng M, Bard J, Mu X. Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Dec;249(12):1514-1528. doi: 10.1002/dvdy.233. Epub 2020 Aug 28. (Medline PMCID: 7855626)
Wu F, Bard JE, Kann J, Yergeau D, Sapkota D, Ge Y, Hu Z, Wang J, Liu T, Mu X. Single cell transcriptomics reveals lineage trajectory of retinal ganglion cells in wild-type and Atoh7-null retinas. Nat Commun. 2021 Mar 5;12(1):1465. doi: 10.1038/s41467-021-21704-4. (Medline PMCID: 7935890)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.