Strain Name:
C57BL/6J-Matr3em3Lutzy/Mmjax
Stock Number:
071285-JAX
Citation ID:
RRID:MMRRC_071285-JAX
Other Names:
C57BL/6J-Matr3^em3Lutzy/Mmjax, MATR3 P154S

Strain Information

Matr3em3Lutzy
Name: matrin 3; endonuclease-mediated mutation 3, Cathleen Lutz
Synonyms: MATR3 P154S
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: CRISPR
Matr3
Name: matrin 3
Synonyms: 2810017I02Rik, 1110061A14Rik, D030046F20Rik
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: CRISPR
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Genetic Alterations
MATR3 P154S knock-in mice carry a proline to serine substitution (P154S) in the Matr3 (matrin 3) locus.
ES Cell Line
Not applicable
Phenotype
Matr3 (matrin 3) encodes a nuclear matrix protein that is involved in RNA processing, binding and stabilizing mRNAs, and modulation DNA damage response. Mutations in Matr3 are associated with familial forms of amyotrophic lateral sclerosis (ALS) and distal myopathy. The P154S mutation in matrin 3 was found in a human ALS and FTD (frontotemporal lobar degeneration) patient. MATR3 P154S knock-in mice were created using CRISPR/cas9 genome editing to insert a proline to serine substitution (P154S) in Matr3. Mice homozygous for the mutation are viable and fertile with normal lifespans. Homozygous MATR3 P154S mice do not exhibit behavioral or motor impairments, neurodegeneration, inflammation, or changes in markers of neurodegeneration or myopathy.
MeSH Terms
  • Animals
  • Mice
  • Amyotrophic Lateral Sclerosis/metabolism
  • Muscles/metabolism
  • Muscular Diseases/genetics
  • Mutation
  • Mutation, Missense
  • Nuclear Matrix-Associated Proteins/genetics
  • Nuclear Matrix-Associated Proteins/metabolism
Strain Development
The MATR3 P154S allele was generated using CRISPR/cas9 endonuclease-mediated genome editing. A guide RNA (5’-TCTCTACCATAACTCAATGT-3’) was selected to target exon 2 of the matrin 3 gene (Matr3) on chromosome 18 and insert a proline to serine substation at amino acid 154 (P154S, CCT to TCT). In addition, the silent nucleotide change G153G (GGC to GGA) is inserted to eliminate the PAM cutting site. The donor plasmid, gRNA and cas9 nuclease were introduced into single cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice (Stock No. 000664) for germline transmission. N1 progeny derived from a single correctly targeted founder carrying the allele were backcrossed to C57BL/6J for at least two generations to establish the colony.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Cat Lutz, Ph.D., The Jackson Laboratory.
Primary Reference

Dominick M, Houchins N, Venugopal V, Zuberi AR, Lutz CM, Meechooveet B, Van Keuren-Jensen K, Bowser R, Medina DX. MATR3 P154S knock-in mice do not exhibit motor, muscle or neuropathologic features of ALS. Biochem Biophys Res Commun. 2023 Feb 19;645:164-172. doi: 10.1016/j.bbrc.2023.01.032. Epub 2023 Jan 13. (Medline PMID: 36689813)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
minimum of N2
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071285-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
071285-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.