Strain Name:
B6C3-Atp1a3em3Lutzy/Mmjax
Stock Number:
071287-JAX
Citation ID:
RRID:MMRRC_071287-JAX
Other Names:
B6C3-Atp1a3^em3Lutzy/Mmjax; Atp1a3^D801N L802L

Strain Information

Atp1a3em3Lutzy
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide; endonuclease-mediated mutation 3, Cathy Lutz
Synonyms: Atp1a3D801N L802L
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: CRISPR
Atp1a3
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms: Atpa-2
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 232975
HGNC: HGNC:801
Homologene: 113729
Genetic Alterations
CRISPR/cas9 genome editing is used to introduce the D801N point mutation in exon 17 of the Atp1a3 gene on chromosome 7. An additional silent nucleotide change was introduced as part of the CRISPR/cas9 targeting strategy. This change of CTG to CTA (L802L) has no effect on protein sequence but could impact potential genotyping strategies.
ES Cell Line
Not applicable
Phenotype
In contrast to C57BL/6 heterozygotes, B6C3 heterozygotes exhibit survival defects with a later onset at ~ 10 weeks of age and ~50% of heterozygous mice reach 35 weeks of age. Nevertheless, heterozygotes still undergo rapid dystonia when submerged in cold water (5°C) at various, tested ages such as 6, 10 and 13 weeks of age. In addition, motor and motor learning defects (rotarod) were observed/tested as early as 10 weeks of age. Of note, heterozygous females demonstrate a better survival compared to heterozygous males.
Strain Development
Plasmids encoding a signal guide RNA designed to introduce the D801N point mutation in exon 17 of the Atp1a3 gene on chromosome 7 and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. An additional silent nucleotide change was introduced as part of the CRISPR/cas9 targeting strategy. This change of CTG to CTA (L802L) has no effect on protein sequence but could impact potential genotyping strategies. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J to develop the C57BL/6J-Atp1a3em3Lutzy/Mmjax (MMRRC Stock No. 066968). Female mice were then bred to C3H/HeJ to develop the colony. This strain is maintained as a cross to B6C3F1/J mice at each generation.
Suggested Control Mice
wild type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Cat Lutz, Ph.D., The Jackson Laboratory.
Primary Reference
Not ready to publish

Colony and Husbandry Information

Heterozygous mice are crossed to B6C3F1/J. Heterozygous mice exhibit sudden and spontaneous deaths. Thus, heterozygous males may be preferred as breeders instead of heterozygous females.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Other or uncertain
Generation
unknown
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071287-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
071287-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.