Strain Detail Sheet

Strain Name:
CC019iPS/UncMmnc
Stock Number:
071424-UNC
Citation ID:
RRID:MMRRC_071424-UNC
Major Collection:
Collaborative Cross iPS Cell Lines

Strain Information

Gene Details

Genetic Alterations
CC019iPS/UncMmnc is an iPS cell line derived from the CC019/Unc mouse strain (MMRRC 066448) which is part of the Collaborative Cross collection of recombinant inbred strains.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    MeSH Terms
    • Animals
    • Breeding
    • Community Networks
    • Crosses, Genetic
    • Databases, Genetic
    • Health Resources
    • Health Services Research
    • Humans
    • Mice
    • Mice, Inbred Strains
    • Recombination, Genetic
    • Chromosome Mapping
    • Genetic Drift
    • Genome/genetics
    • Genotype
    • Haplotypes
    • Male
    • Mice, Inbred Strains/genetics
    • Mutation
    • Polymorphism, Single Nucleotide
    • Quantitative Trait Loci/genetics
    • Extinction, Biological
    • Female
    • Founder Effect
    • Linkage Disequilibrium
    • Reproduction
    Strain Development
    Embryonic Fibroblasts were harvested from sexed E12.5 CC embryos and used for reprogramming using non-integrative technology to obtain iPS cells.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Models for Human Disease
    • Research Tools

    Strain Origin

    Donor
    Terry Magnuson, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Churchill GA, Airey DC, Allayee H, Angel JM, Attie AD, Beatty J, Beavis WD,Belknap JK, Bennett B, Berrettini W, Bleich A, Bogue M, Broman KW, Buck KJ,Buckler E, Burmeister M, Chesler EJ, Cheverud JM, Clapcote S, Cook MN, Cox RD,Crabbe JC, Crusio WE, Darvasi A, Deschepper CF, Doerge RW, Farber CR, Forejt J,Gaile D, Garlow SJ, Geiger H, Gershenfeld H, Gordon T, Gu J, Gu W, de Haan G,Hayes NL, Heller C, Himmelbauer H, Hitzemann R, Hunter K, Hsu HC, Iraqi FA,Ivandic B, Jacob HJ, Jansen RC, Jepsen KJ, Johnson DK, Johnson TE, Kempermann G, Kendziorski C, Kotb M, Kooy RF, Llamas B, Lammert F, Lassalle JM, Lowenstein PR, Lu L, Lusis A, Manly KF, Marcucio R, Matthews D, Medrano JF, Miller DR, MittlemanG, Mock BA, Mogil JS, Montagutelli X, Morahan G, Morris DG, Mott R, Nadeau JH,Nagase H, Nowakowski RS, O'Hara BF, Osadchuk AV, Page GP, Paigen B, Paigen K,Palmer AA, Pan HJ, Peltonen-Palotie L, Peirce J, Pomp D, Pravenec M, Prows DR, QiZ, Reeves RH, Roder J, Rosen GD, Schadt EE, Schalkwyk LC, Seltzer Z, Shimomura K,Shou S, Sillanp MJ, Siracusa LD, Snoeck HW, Spearow JL, Svenson K, TarantinoLM, Threadgill D, Toth LA, Valdar W, de Villena FP, Warden C, Whatley S, WilliamsRW, Wiltshire T, Yi N, Zhang D, Zhang M, Zou F; Complex Trait Consortium. TheCollaborative Cross, a community resource for the genetic analysis of complextraits. Nat Genet. 2004 Nov;36(11):1133-7. (Medline PMID: 15514660)

    Srivastava A, Morgan AP, Najarian ML, Sarsani VK, Sigmon JS, Shorter JR,Kashfeen A, McMullan RC, Williams LH, Giusti-Rodr guez P, Ferris MT, Sullivan P, Hock P, Miller DR, Bell TA, McMillan L, Churchill GA, de Villena FP. Genomes ofthe Mouse Collaborative Cross. Genetics. 2017 Jun;206(2):537-556. doi:10.1534/genetics.116.198838. (Medline PMID: 28592495)

    Collaborative Cross Consortium. The genome architecture of the Collaborative Cross mouse genetic reference population. Genetics. 2012 Feb;190(2):389-401. doi: 10.1534/genetics.111.132639. (Medline PMID: 22345608)

    Colony and Husbandry Information

    Special Considerations

    Cell Line Validation: Cell lines were karyotyped and validated for pluripotency by staining for alkaline phosphatase, pluripotency markers, expression of pluripotency markers by qPCR, and expression of trilineage differentiation by qPCR. See here for more details.

    Reprograming and Thawing Protocols: Click here for detailed information on the protocol used for reprogramming the MEFs to generate iPS cells and protocols for thawing and freezing cells.

    Health Status Report

    Cell lines are routinely tested for mycoplasma

    Order Request Information

    Availability Level

    2 mL vials of cryopreservediPS cells containing >2X106 cells are available to ship withing 2 weeks of order confirmation. iPS cell lines were derived from E12.5 XY, XX embryos, or both, depending on the CC strain.

    Conditions of Distribution

    The Collaborative Cross Conditions of Use can be found here.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071424-UNC-IPS-F CC019iPS/UncMmnc $961.00 / $961.00
    Non-Profit / For-Profit    		 2mL Vial >2X106 cells/vial
    071424-UNC-IPS-M CC019iPS/UncMmnc $961.00 / $961.00
    Non-Profit / For-Profit    		 2mL Vial >2X106 cells/vial
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.