Strain Detail Sheet

Strain Name:
B6J;129S1-Rorbtm1.1Df/Mmucd
Stock Number:
071442-UCD
Citation ID:
RRID:MMRRC_071442-UCD
Other Names:
RORb1-GFP, Rorb1g, RorbGFP

Strain Information

Gene Details

Rorbtm1.1Df
Name: RAR-related orphan receptor beta; targeted mutation 1.1, Douglas Forrest
Synonyms: RorbGFP, Rorb1g
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knock-In
Rorb
Name: RAR-related orphan receptor beta
Synonyms: RZR-beta, Nr1f2, Rorbeta, hstp
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knock-In
NCBI: 225998
Homologene: 38250
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
An enhanced, targeted GFP cDNA replaced the coding sequence in RORβ1-specific exon 1. The final allele lacks the self-excising neomycin resistance cassette inserted downstream of the GFP gene.
ES Cell Line
W9.5/W95

Strain Description

Phenotype
Homozygotes show characteristic gait phenotype referred to as “duck gait”. In the retina, there was a severe loss of horizontal and amacrine cells coupled with increased ganglion cell numbers. This suggests that RORβ1, like Ptf1a, may influence a pathway with alternative differentiation outcomes of ganglion cell versus amacrine and horizontal cells
Mice do not express isoform beta 1, but do express isoform beta 2.

This strain may be useful for studying interneuron differentiation.
MeSH Terms
  • Alleles
  • Amacrine Cells/cytology
  • Amacrine Cells/metabolism
  • Animals
  • Base Sequence
  • Cell Differentiation/genetics
  • Enhancer Elements, Genetic/genetics
  • Gene Deletion
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • Models, Biological
  • Molecular Sequence Data
  • Nuclear Receptor Subfamily 1, Group F, Member 2/genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 2/metabolism
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/metabolism
  • Retinal Horizontal Cells/cytology
  • Retinal Horizontal Cells/metabolism
  • Transcription Factors/genetics
Strain Development
Genomic Rorb 5′ and 3′ homology arms isolated from ES cell (derived from 129/Sv-+p+Tyr-cMgfSl-J/+ mice) DNA were inserted into a pACN vector carrying a self-excisable neomycin-resistance gene (tACE-Cre-Neo). An enhanced gfp cDNA replaced the RORβ1-specific exon. Targeted W9.5 ES cell clones were determined by Southern blot and used to generate chimeric mice. Chimeras were crossed with C57BL/6J mice, the ACN cassette self-excised during germline transmission from male chimeras to yield the Rorb1g allele. Tail-DNA PCR to confirm genotype. After backcrossing for 2 generations onto C57BL/6J, the Rorb+/1g mice intercrossed to generate +/+, +/1g and 1g/1g progeny for analysis.
Suggested Control Mice
wild-type animal of C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Neurobiology
  • Sensorineural

Strain Origin

Donor
Douglas Forrest, Ph.D., NIDDK, NIH.
Hong Liu, Ph.D., NIDDK, NIH.
Primary Reference

Liu H, Kim SY, Fu Y, Wu X, Ng L, Swaroop A, Forrest D. An isoform of retinoid-related orphan receptor β directs differentiation of retinal amacrine and horizontal interneurons. Nat Commun. 2013;4:1813. doi: 10.1038/ncomms2793. (Medline PMID: 23652001)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months Variable
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.