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Strain Detail Sheet

Published: 10/11/2024
Revised: 10/11/2024

Strain Name:
B6J.129S1-Npm1tm1Ppp/Mmucd
Stock Number:
071646-UCD
Citation ID:
RRID:MMRRC_071646-UCD
Other Names:
Npm1 heterozygous

Strain Information

Gene Details

Npm1tm1Ppp
Name: nucleophosmin 1; targeted mutation 1, Pier Paolo Pandolfi
Synonyms: Npm-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
Npm1
Name: nucleophosmin 1
Synonyms: nucleolar protein NO38, B23, NO38
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 18148
HGNC: HGNC:7910
Homologene: 81697

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A targeting vector containing GFP and a modified ACN cassette containing a self-excising neo was inserted in-frame with exon 2, resulting in the removal of exons 2-7.
ES Cell Line
CJ7

Strain Description

Phenotype
Mice develop normally and display no overt lethal phenotypes. Heterozygous mice have hematopoietic phenotypes similar to myelodysplastic syndrome, and older mice have increased risk of cancer can develop hematologic malignancies of both myeloid and lymphoid origin with myeloid malignancies displaying the highest incidence. Homozygous mice are embryonic lethal E11.5-16.5. GFP was demonstrated to express in the same manner as the endogenous protein.
MeSH Terms
  • Animals
  • Apoptosis/genetics
  • Cell Cycle/genetics
  • Cell Transformation, Neoplastic/genetics
  • Cell Transformation, Neoplastic/pathology
  • Cells, Cultured
  • Centrosome/metabolism
  • Embryo Loss/genetics
  • Embryonic Development
  • Fibroblasts
  • Gene Deletion
  • Genomic Instability/genetics
  • Hematopoiesis/genetics
  • In Situ Hybridization, Fluorescence
  • Mice
  • Mice, Knockout
  • Myelodysplastic Syndromes/genetics
  • Myelodysplastic Syndromes/pathology
  • Neoplasms/genetics
  • Neoplasms/metabolism
  • Neoplasms/pathology
  • Nuclear Proteins/deficiency
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Nucleophosmin
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Models for Human Disease

Strain Origin

Donor
Pier Paolo Pandolfi, M.D., Renown Health.
John (Sean) Clohessy, Ph.D., Beth Israel Deaconess Medical Center, Inc..
Primary Reference

Grisendi S, Bernardi R, Rossi M, Cheng K, Khandker L, Manova K, Pandolfi PP. Role of nucleophosmin in embryonic development and tumorigenesis. Nature. 2005 Sep 1;437(7055):147-53. doi: 10.1038/nature03915. Epub 2005 Jul 6. (Medline PMID: 16007073)

Sportoletti P, Grisendi S, Majid SM, Cheng K, Clohessy JG, Viale A, Teruya-Feldstein J, Pandolfi PP. Npm1 is a haploinsufficient suppressor of myeloid and lymphoid malignancies in the mouse. Blood. 2008 Apr 1;111(7):3859-62. doi: 10.1182/blood-2007-06-098251. Epub 2008 Jan 22. (Medline PMCID: 2275037)

Strain Development
Targeting plasmid was linearized and transfected into embryonic stem cells with a 129S1/Sv-Oca2+ Tyr+ Kitl+ background. Homologous recombined embryonic stem cell clones were injected into C57BL/6 blastocysts to generate chimeric progeny. Male chimeras were backcrossed to C57BL/6 females and germline transmission in F1 heterozygous offspring was verified by Southern blot analysis. F1 heterozygotes were interbred to obtain mutant mice, and backcrossed to C57BL/6 mice for greater than 9 generations.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N > 9
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact TVO Director.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact TVO Director

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.