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Strain Detail Sheet

Published: 10/11/2024
Revised: 10/11/2024

Strain Name:
B6J.129S1-Npm1tm1.1Ppp/Mmucd
Stock Number:
071647-UCD
Citation ID:
RRID:MMRRC_071647-UCD
Other Names:
Npm1 Conditional

Strain Information

Gene Details

Npm1
Name: nucleophosmin 1
Synonyms: nucleolar protein NO38, B23, NO38
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18148
HGNC: HGNC:7910
Homologene: 81697

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Conditional ready knockout of the Npm1 gene by floxing exons 1 through 6.
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

Strain Description

Phenotype
None/Normal/Wild-type

Conditional phenotype: Yes. Conditional inactivation in the hematopoietic system leads to loss of hematopoieitic stem cell and phenotypes associated with myelodysplastic syndrome (MDS) and bone marrow failure.

Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Apoptosis
  • Cancer
  • Developmental Biology
  • Hematology
  • Models for Human Disease

Strain Origin

Donor
Pier Paolo Pandolfi, M.D., Renown Health.
John (Sean) Clohessy, Ph.D., Beth Israel Deaconess Medical Center, Inc..
Primary Reference

Nachmani D, Bothmer AH, Grisendi S, Mele A, Bothmer D, Lee JD, Monteleone E, Cheng K, Zhang Y, Bester AC, Guzzetti A, Mitchell CA, Mendez LM, Pozdnyakova O, Sportoletti P, Martelli MP, Vulliamy TJ, Safra M, Schwartz S, Luzzatto L, Bluteau O, Soulier J, Darnell RB, Falini B, Dokal I, Ito K, Clohessy JG, Pandolfi PP. Germline NPM1 mutations lead to altered rRNA 2'-O-methylation and cause dyskeratosis congenita. Nat Genet. 2019 Oct;51(10):1518-1529. doi: 10.1038/s41588-019-0502-z. Epub 2019 Sep 30. (Medline PMCID: 6858547)

Morganti C, Ito K, Yanase C, Verma A, Teruya-Feldstein J, Ito K. NPM1 ablation induces HSC aging and inflammation to develop myelodysplastic syndrome exacerbated by p53 loss. EMBO Rep. 2022 May 4;23(5):e54262. doi: 10.15252/embr.202154262. Epub 2022 Mar 1. (Medline PMCID: 9066051)

Strain Development
The targeting plasmids were linearized and transfected into CJ7 (129S1/Sv-Oca2+ Tyr+ Kitl+) embryonic stem cells and selected using a neomycin resistence casette flanked by FLPase recombination sites. Embryonic stem cell clones were injected into C57BL/6 blastocysts to generate chimeric progeny. Male chimeras were backcrossed to C57BL/6 females. Germline transmission in F1 heterozygous offspring was verified by Southern blot analysis. F1 heterozygotes were interbred with FLPase mice to remove the neomycin casette, and backcrossed with C57BL/6J mice for at least 9 generations before making homozygous conditional mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N > 9
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact TVO Director.

Conditions of Distribution

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact TVO Director

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.