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Strain Detail Sheet

Strain Name:
C57BL/6J-Sf3b1tm2Oaw/Mmucd
Stock Number:
071759-UCD
Citation ID:
RRID:MMRRC_071759-UCD
Other Names:
Sf3b1 R625H conditional knockin

Strain Information

Gene Details

Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: SAP155, Prp10, SF3b155, 2810001M05Rik, Targ4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 81898
Homologene: 6696

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This allele contains an inverted minigene of Sf3b1 exons 12-17 knocked into via homologous recombination.

ES Cell Line
FLP ES Cell Line derived from C57BL/6

Strain Description

Phenotype
None/Normal/Wild-type

Conditional phenotype: Yes, crossing with Mx1-cre recombinase expressing mouse, causes Impaired hematopoiesis and aberrant splicing in blood cells.

MeSH Terms
  • Animals
  • Humans
  • Mice
  • DEAD-box RNA Helicases/genetics
  • DEAD-box RNA Helicases/metabolism
  • HEK293 Cells
  • Hematologic Neoplasms/genetics
  • Hematologic Neoplasms/pathology
  • Hematologic Neoplasms/metabolism
  • Hematopoiesis/genetics
  • Introns
  • Mutation
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • RNA Helicases/genetics
  • RNA Helicases/metabolism
  • RNA Splicing
  • RNA Splicing Factors/genetics
  • RNA Splicing Factors/metabolism
  • RNA-Binding Proteins/genetics
  • RNA-Binding Proteins/metabolism
  • Muscle Proteins/genetics
  • Muscle Proteins/metabolism
Strain Development
Sf3b1 R625H conditional knockin mice were generated using a targeted homologous recombination approach. C57BL/6 FLP embryonic stem (ES) cells were electroporated with linearized targeting constructs encoding inverted exon 12-17 (with R625H mutation) and a FRT-flanked neomycin selection cassette. Surviving clones were expanded for PCR analysis to identify recombinant ES clones. Correctly targeted ES cells were injected into BALB/c blastocysts. Resulting chimeric animals were bred to C57BL/6 wildtype mice to generate germline neo deleted mice.
Suggested Control Mice
wild-type, C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Hematology
  • Models for Human Disease

Strain Origin

Donor
Omar Abdel-wahab, M.D., Memorial Sloan Kettering Cancer Center.
Primary Reference

Benbarche S, Pineda JMB, Galvis LB, Biswas J, Liu B, Wang E, Zhang Q, Hogg SJ, Lyttle K, Dahi A, Lewis AM, Sarchi M, Rahman J, Fox N, Ai Y, Mehta S, Garippa R, Ortiz-Pacheco J, Li Z, Monetti M, Stanley RF, Doulatov S, Bradley RK, Abdel-Wahab O. GPATCH8 modulates mutant SF3B1 mis-splicing and pathogenicity in hematologic malignancies. Mol Cell. 2024 May 16;84(10):1886-1903.e10. doi: 10.1016/j.molcel.2024.04.006. Epub 2024 Apr 29. (Medline PMID: 38688280)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
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