Human NRP1 DNA consisting of CDS (exons 1-17) fused to mouse Nrp1 3'UTR replaced the start codon in mouse Nrp1 gene. This approach minimizes potential downstream splicing or RNP re-cleavage as the guide protospacer was split. Expression of the human CDS is primarily controlled by the mouse regulatory system and is expected to not transcribe the mouse version. CRISPR RNP was used to assist with homologous recombination using dsDNA repair in JM8A3 ES cells. Following blastocyst injection, the chimeras were mated with C57BL/6NCrl to create germline heterozygotes. Long-range PCR products were fully sequenced; potential off-targets were absent.

JM8A3

Unknown

The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.

Littermates of all relevant genotypes.

Kent Lloyd, D.V.M., University of California, Davis.

Not ready to publish

Human NRP1 DNA consisting of CDS (exons 1-17) fused to mouse Nrp1 3'UTR replaced the start codon in mouse Nrp1 gene. This approach minimizes potential downstream splicing or RNP re-cleavage as the guide protospacer was split. Expression of the human CDS is primarily controlled by the mouse regulatory system and is expected to not transcribe the mouse version. CRISPR RNP was used to assist with homologous recombination using dsDNA repair in JM8A3 ES cells. Following blastocyst injection, the chimeras were mated with C57BL/6NCrl to create germline heterozygotes. Long-range PCR products were fully sequenced; potential off-targets were absent. Subsequent backcross was to C57BL/6NCrl.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.