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Strain Detail Sheet

Strain Name:
B6.C3H-Bbaa1CH3/HeNCr/5WjjMmucd
Stock Number:
071885-UCD
Citation ID:
RRID:MMRRC_071885-UCD
Other Names:
B6.C3-Bbaa1 ISRCL5

Strain Information

Gene Details

Bbaa1C3H/HeNCr
Name: B.burgdorferi-associated arthritis 1; C3H/HeNCr
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Non-mutant
Bbaa1
Name: B.burgdorferi-associated arthritis 1
Synonyms: Bb1
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Non-mutant
Cdkn2atm1Cjs
Name: cyclin dependent kinase inhibitor 2A; targeted mutation 1, Charles J Scherr
Synonyms: p19Arf-, ARF-, p19ARF-, p19Arf-null
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Targeted Mutation
Cdkn2a
Name: cyclin dependent kinase inhibitor 2A
Synonyms: MTS1, p19ARF, p16INK4a, INK4a-ARF, p16, ARF-INK4a, Arf, Pctr1, p19ARF, Ink4a/Arf
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Targeted Mutation
NCBI: 12578
HGNC: HGNC:1787
Homologene: 55430

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This C3H/HeNCr-derived Bbaa1 allele confers susceptibility to severe B.burgdorferi-associated arthritis as compared to the allele found in the C57BL/6NCr strain. Commonly referred to as interval-specific recombinant congenic line 5 (ISRCL5), this strain was generated by crossing the Bbaa1C3H/HeNCr QTL variant onto a C57BL/6 genetic background.





ES Cell Line
Not applicable

Strain Description

Phenotype
This advanced congenic mouse line develops severe Lyme arthritis when infected with Borrelia burgdorferi. Arthritis is more severe than the C57BL/6 background. The introgressed region of C3H DNA has been reduced to 2 Mbp (88.3-90.54 Cm of Chr 4), and positional cloning identified an expression level polymorphism in the Cdkn2a encoded gene, p19ARF, as responsible for the increased Lyme arthritis phenotype.
Strain Development
B6.C3-Bbaa1 congenic mice (Chr4: 11.6–93.46 Mbp) were generated by introgression of the Bbaa1C3H/HeNCr QTL variant from C3H onto the B6 background. Continued backcrossing of B6.C3-Bbaa1 mice with B6 mice allowed development of interval-specific recombinant congenic lines (ISRCL). ISRCL5 was generated recently and the interval was fixed by filial mating.

Suggested Control Mice
C57BL/6

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Janis Weis, Ph.D., University of Utah.
Primary Reference

Li J, Ma Y, Paquette JK, Richards AC, Mulvey MA, Zachary JF, Teuscher C, Weis JJ. The Cdkn2a gene product p19 alternative reading frame (p19ARF) is a critical regulator of IFNβ-mediated Lyme arthritis. PLoS Pathog. 2022 Mar 24;18(3):e1010365. doi: 10.1371/journal.ppat.1010365. (Medline PMCID: 8946740)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
complex trait described by JAX

Breeding

MMRRC Breeding System
Sib-mating
Generation
more than 10
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Lucia Irazabal.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Lucia Irazabal

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.