Strain Name:
STOCK Robo1tm1Matl Robo2tm1Mrt/MatlMmmh
Stock Number:
030747-MU
Citation ID:
RRID:MMRRC_030747-MU

Strain Information

Robo1tm1Matl
Name: roundabout guidance receptor 1; targeted mutation 1, Marc Tessier-Lavigne
Synonyms: Robo1gt(PLAP)1Matl, Robo1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Robo2tm1Mrt
Name: roundabout guidance receptor 2; targeted mutation 1, Gail R Martin
Synonyms: Robo2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 19876
Homologene: 2206
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 268902
Homologene: 43188
Genetic Alterations
In the Robo1 allele, the third intron was disrupted by the insertion of a PLAP secretory gene trap vector containing a splice acceptor site followed by Beta-geo, an IRES, placental alkaline phosphatase, and a PGK-1 polyA tail. In the Robo2 allele, The 3' end of exon 1 including the ATG translational start site and part of the following intron were replaced with an IRES-tauLacZ cassette via homologous recombination.
Genotype Determination
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Phenotype
Homozygous: Perinatal lethality. Defective kidney development. Neural defects. Axon guidance defects.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Robo1tm1Matl/Robo1tm1Matl (Genetic Background: involves: CD-1 )

Allelic Composition: Robo1tm1Matl/Robo1tm1Matl (Genetic Background: Not Specified )

Allelic Composition: Robo2tm1Mrt/Robo2tm1Mrt (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Robo2tm1Mrt/Robo2tm1Mrt (Genetic Background: involves: 129P2/OlaHsd * 129/Sv * C57BL/6 * CD-1 )

Allelic Composition: Robo2tm1Mrt/Robo2tm1Mrt (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Robo2tm1Mrt/Robo2tm1Mrt (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Axons/physiology
  • Gene Expression Regulation, Developmental/genetics
  • Glycoproteins/deficiency
  • Glycoproteins/genetics
  • Glycoproteins/physiology
  • Mice
  • Mice, Knockout
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Receptors, Immunologic/deficiency
  • Receptors, Immunologic/genetics
  • Receptors, Immunologic/physiology
  • Spine/embryology
  • Spine/physiology
  • Body Patterning/physiology
  • Cell Differentiation/genetics
  • Embryonic Induction/physiology
  • Glial Cell Line-Derived Neurotrophic Factor
  • Intercellular Signaling Peptides and Proteins
  • Kidney/cytology
  • Kidney/embryology
  • Kidney/metabolism
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Mutation/genetics
  • Nerve Growth Factors/metabolism
  • Signal Transduction/genetics
  • Ureter/cytology
  • Ureter/embryology
  • Ureter/metabolism
Strain Development
No detailed information is available; see original publications. Mixed genetic background designated as STOCK due to contributions from 129P2, 129X1, CD-1, and B6N.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Marc Tessier-Lavigne, Ph.D., Stanford University.
Gail R. Martin, Ph.D., University of California, San Francisco.
Primary Reference

Long H, Sabatier C, Ma L, Plump A, Yuan W, Ornitz DM, Tamada A, Murakami F, Goodman CS, Tessier-Lavigne M. Conserved roles for Slit and Robo proteins in midline commissural axon guidance. Neuron. 2004 Apr 22;42(2):213-23. (Medline PMID: 15091338)

Grieshammer U, Le Ma, Plump AS, Wang F, Tessier-Lavigne M, Martin GR. SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site. Dev Cell. 2004 May;6(5):709-17. (Medline PMID: 15130495)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
MMRRC Breeding System
Sib-mating
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030747-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.