Strain Name:
D2.Cg-Ay/Mmmh
Stock Number:
000137-MU
Citation ID:
RRID:MMRRC_000137-MU
Other Names:
Raly
Major Collection:

Strain Information

Ay
Name: nonagouti; agouti yellow
Synonyms: Ay, A(y)
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: ASP, agouti signal protein, agouti, As
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Genetic Alterations
The spontaneous mutation appears to be a DNA structural alteration that disrupts a gene, hnRNP-associated with lethal yellow (Raly), 5' to the agouti locus and places the agouti locus under the control of the Raly promotor.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
The agouti yellow gene is homozygous lethal. Therefore nonagouti is also segregating. As is true of all DBA mice, these are also mutant at the dilute (Myo5a) and brown (Tyrp1) loci. For this reason, the homozygous nonagouti littermates are dilute brown in phenotype and the heterozygous mice are dilute yellow in phenotype. The full genotype of yellow mice is DBA/2J-Ay/a, Myo5ad/Myo5ad, Tyrp1b/Tyrp1b; the control littermates are DBA/2J-a/a, Myo5ad/Myo5ad, Tyrp1b/Tyrp1b.

Mice heterozygous for the mutant allele have a dilute yellow coat, are obese, but not as obese as C57BL/6J-Ay mice. Yellow is dominant to nonagouti and epistatic to brown. It has not been determined if the yellow mice are prone to diabetes on the DBA background, as are the C57BL/6J-Ay mice.
Mammalian Phenotype Terms
Allelic Composition: Ay/Ay (Genetic Background: 101-Ay )

  • mortality/aging
  • other phenotype
Allelic Composition: Ay/Aw (Genetic Background: 129S1.C3-Ay/Aw Chr 19MOLF/Ei )

Allelic Composition: Ay/a (Genetic Background: B6.Cg-Ay/J )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay/a (Genetic Background: involves: KK )

MeSH Terms
  • Agouti Signaling Protein
  • Alleles
  • Animals
  • Chromosome Mapping
  • Diabetes Mellitus, Type 2/genetics
  • Genes, Dominant/genetics
  • Genes, Lethal/genetics
  • Genes, Recessive/genetics
  • Hair Color/genetics
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mice, Mutant Strains/genetics
  • Muridae/genetics
  • Mutation/genetics
  • Neoplasms, Experimental/genetics
  • Obesity/genetics
  • Proteins/genetics
  • Recombination, Genetic/genetics
  • Sequence Deletion
  • Chromatography, High Pressure Liquid
  • Genotype
  • Glycoproteins/genetics
  • Intramolecular Oxidoreductases/genetics
  • Melanins/chemistry
  • Melanins/genetics
  • Melanocytes/metabolism
  • Membrane Glycoproteins
  • Mice, Congenic
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Models, Chemical
  • Monophenol Monooxygenase/genetics
  • Mutation
  • Oxidoreductases
  • Phenotype
  • Pigmentation/genetics
  • Receptors, Corticotropin/genetics
  • Receptors, Melanocortin
  • Spectrophotometry
  • Agouti-Related Protein
  • Epitopes/metabolism
  • Melanophores/cytology
  • Melanophores/drug effects
  • Melanophores/metabolism
  • Pigmentation/drug effects
  • Pigmentation/physiology
  • Protein Binding
  • Proteins/metabolism
  • Proteins/pharmacology
  • Receptors, Corticotropin/metabolism
  • Receptors, Corticotropin/physiology
  • Recombinant Proteins/genetics
  • Xenopus
  • alpha-MSH/metabolism
  • alpha-MSH/pharmacology
Strain Development
This strain was created by backcrossing the spontaneous mutation from C57BL/6J-Ay/a, that was obtained from JAX, onto DBA/2J.
Suggested Control Mice

Wild-type littermates or DBA/2J mice

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigmentation
  • Obesity
  • Reproduction
Donor
Dr. M. L. Lamoreux, Texas A&M University
Primary Reference
  • Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline PMID: 8146154)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline PMID: 11277491)
  • Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline PMID: 9450927)

Colony and Husbandry Information

Do not feed high fat diet due to obesity and diabetic tendencies. Pups are small and should not be weaned until at least 4-5 weeks of age (the donating investigator routinely waits to wean these mice until they are 8-10 weeks of age). Males seem to mature slowly and don't breed well until they are 3 months of age.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Dilute yellow
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type Female x Heterozygous Male
  • DBA/2J Female x Heterozygous Male
  • Heterozygous females are fertile, but resulting pups have a poor survival rate to weaning age. Recommend mating heterozygous males to DBA/2J or wild-type females.
Generation
N10+
Overall Breeding Performance
Poor. It is usually very much easier to obtain viable litters if yellow males are mated with nonyellow females, and if the mice are maintained in more than usually crowded conditions. Trying to mate these as pairs probably would not work. Some of the yellow females never breed. So in rederivation, it will be necessary to provide more than three animals per client.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
6
Recommended wean age
5 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000137-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000137-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.