Strain Name:
C57BL/6NTac-Il15tm1Imx/TacMmnc
Stock Number:
037398-UNC
Citation ID:
RRID:MMRRC_037398-UNC

Strain Information

Il15tm1Imx
Name: interleukin 15; targeted mutation 1, Immunex Research and Development Corporation
Synonyms: Il15-, IL-15-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Il15
Name: interleukin 15
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 16168
HGNC: HGNC:5977
Homologene: 487
Genetic Alterations
A neomycin resistance cassette replaced 7.5kb of sequence containing exons 3-5, which encode amino acids 1-65.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    IL-15 deficient mice lack natural killer (NK) cells and have marked reductions in memory CD8 T cells, NK1.1 T cells, and Thy1- CD8a intraepithelial lymphocytes (IELs). These mice are unable to mount a protective immune response to challenge with vaccinia virus
    Mammalian Phenotype Terms
    Allelic Composition: Il15tm1Imx/Il15tm1Imx (Genetic Background: C57BL/6-Il15tm1Imx )

    Allelic Composition: Il15tm1Imx/Il15tm1Imx (Genetic Background: C57BL/6NTac-Il15tm1Imx/Tac )

    Allelic Composition: Il15tm1Imx/Il15tm1Imx (Genetic Background: involves: C57BL/6 )

    MeSH Terms
    • Animals
    • CD8-Positive T-Lymphocytes/immunology
    • Cell Lineage
    • Epithelial Cells/immunology
    • Female
    • Immunologic Memory
    • Interleukin-15/genetics
    • Interleukin-15/immunology
    • Killer Cells, Natural/immunology
    • Lymph Nodes/anatomy & histology
    • Lymph Nodes/immunology
    • Lymphocyte Count
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mice, Mutant Strains
    • Organ Size
    • Receptors, Interleukin-15
    • Receptors, Interleukin-2/genetics
    • Receptors, Interleukin-2/immunology
    • Spleen/anatomy & histology
    • Spleen/immunology
    • Thymus Gland/anatomy & histology
    • Thymus Gland/immunology
    • Vaccinia/mortality
    Strain Development
    The disrupted gene was introduced into ES cells of C57BL/6 origin developed at Immunex and injected into BALB/c blastocysts to create chimeric mice. Resulting male chimeric animals were bred to C57BL/6 females and offspring heterozygous for the IL15 knockout allele were crossed to generate IL15 deficient mice (IL15-/-). Different C57BL/6 sublines were used during the development of this line and C57BL/6NTac mice were used as controls in the original studies. Mice were backcrossed an additional 5 times onto C57BL/6NTac mice and intracrossed them to make them homozygous for the IL15 knockout allele.
    Suggested Control Mice
    Wild-type littermates or C57BL/6NTac mice
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Amgen, Inc.
    Primary Reference
    Kennedy MK, Glaccum M, Brown SN, Butz EA, Viney JL, Embers M, Matsuki N, Charrier K, Sedger L, Willis CR, Brasel K, Morrissey PJ, Stocking K, Schuh JC, Joyce S, Peschon JJ. Reversible defects in natural killer and memory CD8 T cell lineages in interleukin 15-deficient mice. J Exp Med. 2000 Mar 6;191(5):771-80. (Medline PMID: 10704459)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Not specified Not specified
    Average litter size
    Not specified
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    037398-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    037398-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    037398-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.