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In the H-2Kb-/- mouse, no cell surface expression of Kb on peripheral lymphocytes can be detected on analysis by immunofluorescence while similar analysis reveals a compensatory increase in cell surface expression of other Class I molecules (Db, Q7, and Q9) and almost unaltered levels of b2m as compared to normal B6 mice. However, a substantial reduction (30-50%) of peripheral CD8ab+ T-cells was observed with no accompanying alteration in Vb diversity. The mice are useful for studying cytolytic T lymphocyte education and mobilization against pathogens and the analysis of the role of MHC restriction in various diseases.
C57BL/6 mice deficient in Kb were generated by homologous recombination in the laboratory of Dr. Francois Lemonnier of The Institut Pasteur, Paris France. Generation of H-2 Kb Targeted Mutation mice-The Kb gene was disrupted by replacing the 2nd and 3rd exons with an HPRT minigene inserted in the opposite transcriptional orientation. The disrupted gene was introduced into an E14TGa (H-2b and b2ma) HPRT deficient embryonic stem cell of 129P2/OlaHsd origin. After injection into B6 blastocysts, the resultant germline chimeras were backcrossed twice to C57BL/6J mice and offspring heterozygous for the H2-Kb knockout allele were crossed to generate H2-Kb deficient mice (Kb -/-). Inactivation of the H2-Kbgene was confirmed by Southern blot analysis on tail DNA and by FACS analysis. The targeted mutation mice were subsequently backcrossed to C57BL/6J mice another 10 times before being intercrossed to achieve homozygosity. Six pairs of the N12 homozygous mice were transferred from Dr. Lemonnier to the NIAID repository at Taconic in 2001 where they were embryo transfer derived. The MMRRC received the model thereafter.
Pérarnau B, Saron MF, Reina San Martin B, Bervas N, Ong H, Soloski MJ, SmithAG, Ure JM, Gairin JE, Lemonnier FA. Single H2Kb, H2Db and double H2KbDb knockoutmice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitisvirus cytolytic responses. Eur J Immunol. 1999 Apr;29(4):1243-52. (Medline PMID: 10229092)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.