Strain Name:
B6J.129P2-Anp32tm1Mak/Mmjax
Stock Number:
044045-JAX
Citation ID:
RRID:MMRRC_044045-JAX
Other Names:
ANP32B B6F10

Strain Information

Anp32btm1Mak
Name: acidic nuclear phosphoprotein 32 family member B; targeted mutation 1, Tak Mak
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knock-In
Anp32b
Name: acidic nuclear phosphoprotein 32 family member B
Synonyms: PAL31, 2410015B15Rik, PHAPI2a
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knock-In
NCBI: 67628
Homologene: 137872
Genetic Alterations
Anp32b exons 2,3 and 4 deletion with Neo insertion in reverse orientation.
Genotype Determination
ES Cell Line
E14K derived from 129P2/OlaHsd
Phenotype
Anp32b is very important for murine embryogenesis. In a mixed genetic background, Anp32b-deficient mice displayed a partially penetrant perinatal lethality that became fully penetrant in a pure C57BL/6 background. Surviving Anp32b-deficient mice showed reduced viability due to variable defects in various organ systems. Study of compound mutants lacking Anp32a, Anp32b, and/or Anp32e revealed previously hidden roles for Anp32a in mouse development that became apparent only in the complete absence of Anp32b.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Apoptosis
  • Biomarkers, Tumor/metabolism
  • Breast Neoplasms/metabolism
  • Breast Neoplasms/pathology
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/metabolism
  • Cell Proliferation
  • Cells, Cultured
  • Embryo, Mammalian/anatomy & histology
  • Embryo, Mammalian/physiology
  • Female
  • Fibroblasts/cytology
  • Fibroblasts/physiology
  • Gene Targeting
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Survival Rate
Strain Development
The Anp32b gene was disrupted by replacing the genomic region containing exons 2, 3, and 4 with a pgk-neo expression cassette in reverse orientation. This mutation results in an mRNA in which the last 17 codons of exon 1 are linked to an out-of-frame exon 5. Anp32b+/- ES cell lines (E14K) were obtained by homologous recombination and microinjected into C57BL/6 blastocysts. The resulting chimeras were bread into C57BL/6 mice from Jackson and backcrossed for 10 generations (JAX Strain #000664)
Suggested Control Mice
Anp32b+/+ and Anp32b+/-
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
Donor
Tak Mak, Ph.D Campbell Family Institute for Breast Cancer Research
Primary Reference

Reilly PT, Afzal S, Gorrini C, Lui K, Bukhman YV, Wakeham A, Haight J, LingTW, Cheung CC, Elia AJ, Turner PV, Mak TW. Acidic nuclear phosphoprotein 32kDa(ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammaliandevelopment. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10243-8. doi:10.1073/pnas.1106211108. Epub 2011 Jun 2. (Medline PMID: 21636789)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
Less than 4

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044045-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
044045-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.