Strain Name:
Crl:CD1-Neurog2tm3(Ascl1)Fgu/Mmucd
Stock Number:
050508-UCD
Citation ID:
RRID:MMRRC_050508-UCD
Other Names:
Neurog2tm3(Ascl1)Fgu, Ngn2Mash1/KI

Strain Information

Neurog2tm3(Ascl1)Fgu
Name: neurogenin 2; targeted mutation 3, Francois Guillemot
Synonyms: Ngn2KIMash1, Ngn2KIM1, Ngn2Mash1KI
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knock-In
Neurog2
Name: neurogenin 2
Synonyms: Math4A, ngn2, Atoh4, bHLHa8
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knock-In
NCBI: 11924
Homologene: 7633
Ascl1
Name: achaete-scute family bHLH transcription factor 1
Synonyms: ASH1, Mash1, bHLHa46
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knock-In
NCBI: 17172
HGNC: HGNC:738
Homologene: 31234
Genetic Alterations
The transcription factor Ascl1 coding region replaces that of Neurog2 using homologous recombination. The late embryonic lethality of loss of Neurog2 is not rescued in homozygotes
Genotype Determination
ES Cell Line
R1
Phenotype
Heterozygotes are normalHomozygotes die at birth from defects in thalamus that affect feeding behavior
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • nervous system
MeSH Terms
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Lineage
  • DNA Primers
  • DNA-Binding Proteins/genetics
  • Down-Regulation
  • Gene Expression Regulation, Developmental
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Neurons/cytology
  • Telencephalon/cytology
  • Telencephalon/embryology
  • Transcription Factors/genetics
Strain Development
The Neurog2Mash1/KI targeting vector was designed by replacing the bHLH domain of Neurog2 with Mash1-coding sequences, leaving 48 amino acids of the aminoterminal Neurog2 coding sequence. The construct was electroporated into R1 ES cells. Homologous recombination was assayed by genomic Southern blotting. Chimeras were mated to CD1 mice to propagate the mutation through the germline and subsequently expand the line.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Neurobiology
  • Sensorineural
Donor
Francois Guillemot, Ph.D The Francis Crick Institute
Primary Reference

Fode C, Ma Q, Casarosa S, Ang SL, Anderson DJ, Guillemot F. A role for neural determination genes in specifying the dorsoventral identity of telencephalicneurons. Genes Dev. 2000 Jan 1;14(1):67-80. (Medline PMID: 10640277)

Maurer KA, Kowalchuk A, Shoja-Taheri F, Brown NL. Integral bHLH factorregulation of cell cycle exit and RGC differentiation. Dev Dyn. 2018Aug;247(8):965-975. doi: 10.1002/dvdy.24638. Epub 2018 Jun 26. (Medline PMID: 29770538)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Generation
N = > 30 generations on CD1 background. Maintained by N. Brown (UC Davis) on CD1 from 2005 until donation to MMRRC in 2018.
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 8 to 12 months
Average litter size
10-14
Recommended wean age
3 Weeks
Average Pups Weaned
10-14

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050508-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
050508-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.