Strain Name:
C3Fe.129-Sox10tm1Weg/Mmnc
Stock Number:
050522-UNC
Citation ID:
RRID:MMRRC_050522-UNC
Other Names:
C3Fe.Sox10tm1Weg, Sox10-, Sox10lacZ

Strain Information

Sox10tm1Weg
Name: SRY (sex determining region Y)-box 10; targeted mutation 1, Michael Wegner
Synonyms: Sox10-, Sox10LacZKO, Sox10lacZ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knockout
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: Sox21, gt
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Knockout
NCBI: 20665
VEGA: 15
Homologene: 5055
Genetic Alterations
The allele provides an in-frame insertion of a lacZ-neomycin resistance cassette replacing the Sox10 coding sequences. RT-PCR studies did not detect wild-type transcript in homozygous E12.5 embryos.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    When congenic on the C3HeB/FeJ strain, heterozygotes do not exhibit aganglionic megacolon, and are readily distinguished from littermates by spotting that is observed as a variable sized belly spot and consistent white fee. Homozygous embryos can be observed at 13.5 and 14.5dpc on this strain background.
    Mammalian Phenotype Terms
    Allelic Composition: (Genetic Background: )

    MeSH Terms
    • Animals
    • Cell Differentiation
    • Chimera
    • DNA-Binding Proteins/genetics
    • DNA-Binding Proteins/metabolism
    • Ganglia, Spinal/embryology
    • Gene Expression Regulation, Developmental
    • Heterozygote
    • High Mobility Group Proteins/genetics
    • High Mobility Group Proteins/metabolism
    • Homozygote
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Mice, Transgenic
    • Neural Crest/cytology
    • Neuroblastoma
    • Neuroglia/cytology
    • Neuroglia/physiology
    • Rats
    • Receptor, ErbB-3/genetics
    • SOXE Transcription Factors
    • Transcription Factors/genetics
    • Transcription Factors/metabolism
    • Tumor Cells, Cultured
    • beta-Galactosidase/genetics
    Strain Development
    This strain was developed by Dr. Michael Wegner as described in PMID:11156606. A targeting event was engineered that inserted a LacZ reporter in frame with the endogenous ATG and deleted the coding sequences of the Sox10 gene. Chimeras were initially bred to C3HeB/FeJLe-a/a to obtain viable animals for at least 4 generations. Subsequently, they were backcrossed to C3HeB/FeJ for more than 20 generations and maintained by repeatedly backcrossing to C3HeB/FeJ.
    Suggested Control Mice
    wild-type C3HeB/FeJ littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology
    Donor
    Michael Wegner, Ph.D., Institut für Biochemie, FAU Erlangen-Nürnberg.
    Michelle Southard-Smith, Ph.D., Vanderbilt University.
    Primary Reference

    Britsch S, Goerich DE, Riethmacher D, Peirano RI, Rossner M, Nave KA,Birchmeier C, Wegner M. The transcription factor Sox10 is a key regulator ofperipheral glial development. Genes Dev. 2001 Jan 1;15(1):66-78. PubMed PMID:11156606; PubMed Central (Medline PMCID: PMC312607)

    Colony and Husbandry Information

    Avoid breeding onto C57BL/6J or 129 strains as these genetic backgrounds will increase severity of aganglionic megacolon leading to increased death of the heterozygous Sox10-LacZ animals.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Agouti
    Eye
    Black
    Other
    While other Sox10 knockout alleles have been made, none have been made congenic on C3HeB/FeJ like this strain. Being on this genetic background suppresses aganglionic megacolon in heterozygotes and increases survival of homozygous fetuses so additional studies of the effect of Sox10 loss on a variety of tissues are possible.
    MMRRC Breeding System
    Backcross
    Generation
    N20+ (C3HeB/FeJ)
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No
    Homozygotes are fertile: N/A N/A
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months 6 to 8 months
    Average litter size
    7-9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    5-9

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    050522-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    050522-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    050522-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.