Strain Detail Sheet

Strain Name:
129S(Cg)-Krastm1.1Cmco/Mmnc
Stock Number:
050601-UNC
Citation ID:
RRID:MMRRC_050601-UNC
Other Names:
Kras ex3op/ex3op

Strain Information

Gene Details

Krastm1.1Cmco
Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1.1, Christopher M Counter
Synonyms: Krasex3op
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Kras
Name: Kirsten rat sarcoma viral oncogene homolog
Synonyms: Kras-2, Kras2, Ki-ras, K-ras
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 16653
HGNC: HGNC:6407
Homologene: 37990
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
33 synonymous mutations in Kras exon 3, converting 27 rare codons into more common codons.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not specified

    Strain Description

    Phenotype
    Homozygous for a codon-altered Kras knock-in allele that contains 33 synonymous mutations in Kras exon 3, converting 27 rare codons into more common codons. These mice don’t have an overt developmental phenotype, even though the average amount of Kras protein is increased in tested mouse tissues.
    MeSH Terms
    • Adenoma/chemically induced
    • Adenoma/genetics
    • Adenoma/pathology
    • Animals
    • Carcinogenesis/genetics
    • Cell Proliferation
    • Cells, Cultured
    • Codon
    • Female
    • Humans
    • Lung Neoplasms/chemically induced
    • Lung Neoplasms/genetics
    • Lung Neoplasms/pathology
    • Male
    • Mice, 129 Strain
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Proto-Oncogene Proteins p21(ras)/genetics
    • Tumor Burden
    • Urethane
    Strain Development
    A knock-in construct was created using long and short arms of homology from a 129S7/AB2.2 genomic mouse BAC and a synthetic exon 3, in which all rare codons were converted into one of the two most commonly used codons in the mouse genome, provided they were not located in annotated or sites likely to affect splicing. Targeting of the Kras allele in 129S6-Taconic ES cells and blastocyst injection were performed using standard procedures. Chimeras were crossed with 129S6/SvEvTac mice and progeny were screened for germline transmission by genotyping PCR. Following germline transmission, mice were crossed with 129S/Sv-Tg(Prm-cre)58Og/J mice (JAX Strain #003328) that express Cre recombinase in the male germline. Male animals expressing the mutant allele and Cre were crossed with 129 females to generate progeny with confirmed excision of the neo cassette and lacking the Prm-driven Cre transgene. These mice were intercrossed to generate homozygotes.
    Suggested Control Mice
    wild-type 129S mice

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Hematology

    Strain Origin

    Donor
    Christopher Counter, Ph.D., Duke University.
    Primary Reference

    Pershing NL, Lampson BL, Belsky JA, Kaltenbrun E, MacAlpine DM, Counter CM.Rare codons capacitate Kras-driven de novo tumorigenesis. J Clin Invest. 2015Jan;125(1):222-33. doi: 10.1172/JCI77627. Epub 2014 Dec 1. (Medline PMID: 25437878)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Agouti
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    050601-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.