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Availability & Fees Order this Strain
In mouse, the two members of the Mageb5 gene family exist on the X chromosome adjacent to each other in inverted segmental duplications (palindrome). The donating investigators had previously deleted one copy of the gene family (Del(XMageb5)1Jbmu). In this strain, they deleted the second copy as well (Mageb5bem1Jbmu).
The donating investigators used CRISPR to delete the first Mageb5 family member in B6SJLF1/J zygotes. The resulting mice were bred to C57BL/6J females to keep the deletion within a B6J chromosomal segment. Mice were further backcrossed to B6J.
To generate a complete Mageb5 knockout line, zygotes from these mice were used to perform yet another CRISPR manipulation, targeting the second gene family member. The resulting “L1”mouse line contains an ~860 bp deletion in the second Mageb5 family member. Mice were further backcrossed to B6J males for at least 10 generations.
The donating investigators used CRISPR to delete the first Mageb5 family member in B6SJLF1/J zygotes. The resulting mice were bred to C57BL/6J females to keep the deletion within a B6J chromosomal segment. Mice were further backcrossed to B6J. Zygotes from this line were used to perform yet another CRISPR manipulation, targeting the second gene family member. The resulting “L1”mouse line contains an ~860 bp null deletion in the second Mageb5 family member. Mice were further backcrossed to B6J males for at least 10 generations.
Stark-Dykema ER, Dulka EA, Gerlinger ER, Mueller JL. X-linked palindromic gene families 4930567H17Rik and Mageb5 are dispensable for male mouse fertility. Sci Rep. 2022 May 20;12(1):8554. doi: 10.1038/s41598-022-12433-9. (Medline PMID: 35595785)
Kruger AN, Ellison Q, Brogley MA, Gerlinger ER, Mueller JL. Male mice with large inversions or deletions of X-chromosome palindrome arms are fertile and express their associated genes during post-meiosis. Sci Rep. 2018 Jun 12;8(1):8985. doi: 10.1038/s41598-018-27360-x. (Medline PMID: 29895860)
Colony Surveillance Program and Current Health Reports
The strain donors report that mice lacking these two Mage5b family members do not have detectable defects in male fertility, fecundity, spermatogenesis, or in gene regulation.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact John Corthell.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact John Corthell
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.