Strain Name:
B6J.B6N(Cg)-Poglut2tm2d(EUCOMM)Hmgu/RshMmunc
Stock Number:
071405-UNC
Citation ID:
RRID:MMRRC_071405-UNC
Other Names:
Poglut2 tm2D
Major Collection:

Strain Information

Poglut2tm2d(EUCOMM)Hmgu
Name:
Type:
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Poglut2
Name: protein O-glucosyltransferase 2
Synonyms: EP58, 1810049A15Rik, 5730416C13Rik, Kdel1, Kdelc1
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 72050
Homologene: 11367
Genetic Alterations
Targeted (Conditional ready, Null/knockout, Reporter). The L1L2_Bact_P cassette was inserted upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s). The critical exon(s) is/are thus flanked by loxP sites.

The Poglut2 tm2C allele was generated using the mouse codon optimized FLP (FLPo) flippase (B6.129S4-Gt(ROSA)26Sor tm2(FLP*)Sor /J (https://www.jax.org/strain/012930). Subsequently, the Poglut2 tm2D allele, the global null allele, was generated using Cre-recombinase (https://www.jax.org/strain/006054).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    JM8A3.N1 derived from C57BL/6N
    Phenotype
    Poglut2 tm2D KO homozygotes, in conjunction with Poglut3 KO homozygotes, have severely reduced body size and viability, and soft tissue syndactyly due to elimination of O-glucosylation on specific EGFs. Homozygous animals of this strain appear to need at least one wild type copy of Poglut3 in order to be phenotypically normal.

    Poglut2 tm2D heterozygous animals are viable, fertile, and appear phenotypically normal.
    Strain Development
    EUCOMM-targeted knockout-first allele (tm2A) from IKMC project 74684 was electroporated into JM8A3.N1 ES cells. Clone HEPD0770_7_B12 was used for microinjection into C57BL/6N blastocysts and PCR confirmed chimera were mated to C57BL/6N females for germline derived F1 mice. F1 mice carrying the Poglut2 tm2A allele were confirmed by PCR. These heterozygous Poglut2 tm2A carriers were then mated to FLP (FLPo) flippase (B6.129S4-Gt(ROSA)26Sor tm2(FLP*)Sor /J; common name: ROSA26Flpo)(https://www.jax.org/strain/012930) to create the Poglut2 tm2C global conditional ready (floxed); reporter and neo removed allele.

    Poglut2 tm2C heterozygotes, confirmed by PCR, were crossed to CMV-cre (B6.C-Tg(CMV-cre)1Cgn/J) (https://www.jax.org/strain/006054) to generate the Poglut2 tm2D global knockout null allele. Poglut2 tm2D carriers, confirmed by PCR, were crossed to C57BL/6J (JAX strain 00664) for 11+ generations.
    Suggested Control Mice
    C57BL/6J (JAX strain 000664)
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
    Donor
    Robert Haltiwanger, Ph.D., University of Georgia.
    Primary Reference
    In preparation, submitted, or in press

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross
    Generation
    11+
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    071405-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.