JM8A3 derived from C57BL/6N

Heterozygous animals are viable, appear phenotypically normal, and are fertile.The phenotype of homozygous animals is unknown.

The PG00252_Z_1_B04 targeting vector Poglut3tm380258 was inserted upstream of exon 2 in the Poglut3 locus on chromosome 9. The cassette is composed of an FRT site followed by a splice acceptor, IRES, lacZ, polyA site, a loxP site, a neomycin resistance gene, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3. Dr. Robert Haltiwanger (University of Georgia) obtained the targeting vector from EuMMCR (IKMC Project 101611) and had it introduced into JM8A3 embryonic stem (ES) cells and checked for correct targeting at Texas A&M Institute for Genomic Medicine. ES cells were injected into blastocysts, the resulting chimeric males were bred to C57BL/6N females, and Poglut3 tm1Rsh (knockout first) progeny were crossed to B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J (JAX Stock No. 012930) to remove the lacZ reporter and neomycin cassette at the Mouse Transgenic and Gene Targeting Core at Emory University.

C57BL/6J

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

Robert Haltiwanger, Ph.D., University of Georgia.

In preparation, submitted, or in press