Strain Detail Sheet

Strain Name:
B6J.B6N(Cg)-Poglut3tm1.3Rsh/Mmunc
Stock Number:
071408-UNC
Citation ID:
RRID:MMRRC_071408-UNC
Other Names:
Poglut3 tm1.3

Strain Information

Gene Details

Poglut3
Name: protein O-glucosyltransferase 3
Synonyms: 4833410J10Rik, 2010004J24Rik, Kdelc2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68304
VEGA: 9
Homologene: 17805
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Genetic Alterations
Poglut3 targeting vector was purchased from EuMMCR. Poglut3 tm1a heterozygous ES cells were generated using ES cell line: Nagy G4 (129/SvEvBrd x C57BL/6); JM8A3 (C57BL/6N).
Animals heterozygous for the Poglut3 tm1A allele were generated by injecting ES cells into C57BL/6N blastocysts, and resultant chimeras were mated to females of the same strain of mice to generate animals heterozygous for the Knockout First allele.

Poglut3 tm1c alleles were generated using the mouse codon optimized FLP (FLPo) flippase (B6.129S4-Gt(ROSA)26Sor tm2(FLP*)Sor /J. Subsequently, the Poglut3 tm1.3 allele was generated using Cre-recombinase and is the global null allele.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8A3 derived from C57BL/6N

    Strain Description

    Phenotype
    Poglut3 tm1.3 KO homozygotes, in conjunction with Poglut2 KO homozygotes, have severely reduced body size and viablility, and soft tissue syndactyly due to elimination of O-glucosylation on specific EGFs.

    Homozygous animals of this strain appear to need at least one wild type copy of Poglut2 in order to be phenotypically normal.

    Poglut3 tm1.3 heterozygous animals are viable, fertile, and appear phenotypically normal.

    Strain Development
    The PG00252_Z_1_B04 targeting vector Poglut3tm380258(L1L2_Bact_P) was inserted upstream of exon 2 in the Poglut3 locus on chromosome 9. The cassette is composed of an FRT site followed by a splice acceptor, IRES, lacZ, polyA site, a loxP site, a neomycin resistance gene, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3. Dr. Robert Haltiwanger (University of Georgia) obtained the targeting vector from EuMMCR (IKMC Project 101611) and had it introduced into JM8A3 embryonic stem (ES) cells and checked for correct targeting at Texas A&M Institute for Genomic Medicine. ES cells were injected into blastocysts, the resulting chimeric males were bred to C57BL/6N females, and Poglut3 tm1Rsh (knockout first) progeny were crossed to B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J (JAX Stock No. 012930) to remove the lacZ reporter and neomycin cassette at the Mouse Transgenic and Gene Targeting Core at Emory University. These mice carrying the Poglut3 tm1.2Rsh allele were then breed to B6.C-Tg(CMV-cre)1Cgn/J (Common name: CMV-cre); JAX strain 006054, to remove Poglut3 exons 2 and 3 to make this Poglut3 tm1.3Rsh global null allele.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease

    Strain Origin

    Donor
    Robert Haltiwanger, Ph.D., University of Georgia.
    Primary Reference
    In preparation, submitted, or in press

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    N=12
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071408-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.