Strain Detail Sheet

Strain Name:
C57BL/6J-Cbx2em2Rek/Mmmh
Stock Number:
071608-MU
Citation ID:
RRID:MMRRC_071608-MU
Other Names:

Cbx2d46, Cbx2Δ

Strain Information

Gene Details

Cbx2
Name: chromobox 2
Synonyms: M33
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12416
HGNC: HGNC:1552
Homologene: 7256
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Genetic Alterations

The Cbx2Δ strain has a CRISPR/Cas9 deletion (mm10, chr11:119,028,119–119,028,164) in the Cbx2 gene that introduces a premature stop codon after amino acid 17.

ES Cell Line
Not applicable

Strain Description

Phenotype

Mice homozygous for the Cbx2Δ mutation display preweaning lethality (100% penetrance). Average litter size for heterozygotes when backcrossed to C57BL6/J was 6.5 (median 7, stdev 2.1, 27 litters).

MeSH Terms
  • Animals
  • Male
  • Chromatin/metabolism
  • Cell Nucleus/metabolism
  • Polycomb Repressive Complex 1/genetics
  • Polycomb Repressive Complex 1/metabolism
  • Polycomb-Group Proteins/genetics
  • Polycomb-Group Proteins/metabolism
  • Spermatogenesis/genetics
Strain Development
The single-guide RNA (sgRNAs) pair, Cas9 nickase mRNA, and plasmid donor for homologous recombination were injected into mouse zygotes (C57BL/6 inbred strain) by the Genome Modification Facility at Harvard University. Mice born from the injected zygotes were screened for homologous recombination events via PCR-RFLP (restriction fragment length polymorphism). The Cbx2Δ strain has a deletion (mm10, chr11:119,028,119–119,028,164) by CRISPR/Cas9 that introduces a premature stop codon after amino acid 171 without repair by homologous recombination donor. The Cbx2Δ strain has been maintained by backcrossing to C57BL/6J at least 17 times from 2016 to 2023.
Suggested Control Mice
C57BL/6J or siblings (for HET to WT comparison or HOMO to WT comparison for embryos)

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Reproduction

Strain Origin

Donor
Robert Kingston, Ph.D., Massachusetts General Hospital.
Primary Reference

Kim JJ, Steinson ER, Lau MS, de Rooij DG, Page DC, Kingston RE. Cell type-specific role of CBX2 and its disordered region in spermatogenesis. Genes Dev. 2023 Jul 1;37(13-14):640-660. doi: 10.1101/gad.350393.122. Epub 2023 Aug 8. (Medline PMID: 37553262)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
17
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
6.5
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.