Strain Name:
C57BL/6NCrl-Pigylem1(IMPC)Mbp/Mmucd
Stock Number:
071635-UCD
Citation ID:
RRID:MMRRC_071635-UCD
Other Names:
CR11457
Major Collection:

Strain Information

Pigylem1(IMPC)Mbp
Name: phosphatidylinositol glycan anchor biosynthesis, class Y-like; endonuclease-mediated mutation 1, Mouse Biology Program
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Pigyl
Name: phosphatidylinositol glycan anchor biosynthesis, class Y-like
Synonyms: 1810008A14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66268
Homologene: 134660
Genotype Determination
Phenotype
Phenotyping data may be available at mousephenotype.org.
Strain Development
CRISPR guide(s) and Cas9 protein were microinjected or electroporated into C57BL/6NCrl zygotes and progeny were screened for the desired mutation. Founders were mated to C57BL/6NCrl breeders, and derived N1 progeny were identified by PCR and/or sequencing. N1 were then mated again to C57BL/6NCrl breeders, to generate N2 mice, identified by PCR and/or sequencing. N2 heterozygous mutant mice were mated for production of phenotyping cohorts, and N2 or N2F1 mice were used for cryopreservation purposes.
Suggested Control Mice
C57BL/6NCrl or wild-type from colony
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Kent Lloyd, D.V.M., University of California, Davis.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Order Request Information

The availability level for this product has not been determined.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A license from third party patent owner(s) may be required for for-profit entities to use mouse models derived from CRISPR-Cas9 technologies and it is the Users sole responsibility to determine whether such a license is necessary.

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