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Strain Detail Sheet

Strain Name:
B6J.B6NCrl-Fzd2em1Rstot/Mmmh
Stock Number:
071682-MU
Citation ID:
RRID:MMRRC_071682-MU
Other Names:
Fzd2-D3 ; Fzd2em1Rstot(D3). MGI:7450775

Strain Information

Gene Details

Fzd2em1Rstot
Name: frizzled class receptor 2; endonuclease-mediated mutation 1, Rolf Stottmann
Synonyms: Fzd2em1Rstot(D3), Fzd2D3
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: CRISPR
Fzd2
Name: frizzled class receptor 2
Synonyms: Mfz10a, Mfz10, Fz10
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: CRISPR
NCBI: 57265
HGNC: HGNC:4040
Homologene: 20377

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This strain harbors an allele that consists of a 3 bp CRISPR-generated deletion that targets a serine at position 552.

ES Cell Line
Not applicable

Strain Description

Phenotype
Mice that are homozygous for this allele display a cleft palate (incompletely penetrant) and shortened long bones
MeSH Terms
  • Animals
  • Humans
  • Mice
  • Cleft Palate/genetics
  • Dwarfism/genetics
  • Limb Deformities, Congenital/genetics
  • Urogenital Abnormalities/genetics
  • Wnt Signaling Pathway/genetics
  • Disease Models, Animal
  • Frizzled Receptors/genetics
  • Gene Knock-In Techniques
Strain Development
CRISPR guide(s) and Cas9 protein were microinjected into C57BL/6NCrl zygotes and progeny were screened for the desired mutation. Founders were mated to C57BL/6J breeders (Jackson Labs Stock #000664), and derived N1 progeny were identified by PCR and sequencing. N1 were then mated again to C57BL/6J breeders to generate N2 mice identified by PCR and sequencing. N2 heterozygous mutant mice were mated for production of phenotyping cohorts, and >N8 mice were used for cryopreservation purposes.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease

Strain Origin

Donor
Rolf Stottmann, Ph.D., Nationwide Children's Hospital.
Primary Reference

Liegel RP, Michalski MN, Vaidya S, Bittermann E, Finnerty E, Menke CA, Diegel CR, Zhong ZA, Williams BO, Stottmann RW. Successful therapeutic intervention in new mouse models of frizzled 2-associated congenital malformations. Development. 2023 Feb 1;150(3):dev201038. doi: 10.1242/dev.201038. Epub 2023 Feb 15. (Medline PMID: 36789910)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Reduced Reduced
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Andrew Corris.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Andrew Corris

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.