SV40TAg is expressed in all mesothelial cells in MexTAg mice to varying levels depending on the MexTAg line. Low level SV40 TAg expression (266sHom and 270iHom) confers minimal phenotypic changes relative to wild type C57BL/6 mice. Mice carrying high copy numbers of SV40 TAg (304Hom; 64 copies and 299Het 97 copies) have moderate to severe phenotype changes. High copy number mice exhibit thin fur and a hunched back. They are also more prone to abscesses, ear infection, spontaneous tumors, limb, neck, or chest swelling. 299Het mice are prone to anal prolapse and skin tags. This phenotype is less pronounced in lower copy number mice (266sHom and 270iHom). Heterozygous lines for 266, 270 and 304 display less severe phenotypes. However, these are not actively bred. All lines except 299h can be maintained as homozygotes. Upon asbestos exposure, 83%-100% of MexTAg mice will develop mesothelioma, with few, if any non-mesothelioma tumors. Minimal tumor development is observed in the absence of asbestos.
Robinson C, van Bruggen I, Segal A, Dunham M, Sherwood A, Koentgen F, Robinson BW, Lake RA. A novel SV40 TAg transgenic model of asbestos-induced mesothelioma: malignant transformation is dose dependent. Cancer Res. 2006 Nov 15;66(22):10786-94. doi: 10.1158/0008-5472.CAN-05-4668. (Medline PMID: 17108115)
Robinson C, Walsh A, Larma I, O'Halloran S, Nowak AK, Lake RA. MexTAg mice exposed to asbestos develop cancer that faithfully replicates key features of the pathogenesis of human mesothelioma. Eur J Cancer. 2011 Jan;47(1):151-61. doi: 10.1016/j.ejca.2010.08.015. Epub 2010 Sep 16. (Medline PMID: 20850297)
Robinson C, Dick IM, Wise MJ, Holloway A, Diyagama D, Robinson BW, Creaney J, Lake RA. Consistent gene expression profiles in MexTAg transgenic mouse and wild type mouse asbestos-induced mesothelioma. BMC Cancer. 2015 Dec 18;15:983. doi: 10.1186/s12885-015-1953-y. (Medline PMID: 26680231)
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.Viability and Fertility: | Female | Male | Comments |
---|---|---|---|
Homozygotes are viable: | Yes | Yes | |
Homozygotes are fertile: | Yes | Yes | |
Hetero/Hemizygotes are fertile: | Yes | Yes | |
Age Reproductive Decline: | Undetermined | Undetermined |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Jenette Creaney.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Jenette Creaney
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
---|---|---|---|---|
071903-MU-SPERM | Cryo-preserved spermatozoa | $437.00 / $817.00 Non-Profit / For-Profit |
Aliquot | Approximate quantity3 |
071903-MU-RESUS | Litter recovered from cryo-archive | $2,624.00 / $5,340.00 Non-Profit / For-Profit |
Litter | Recovered litter4; additional fees for any special requests. |
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information. |
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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