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Strain Detail Sheet

Strain Name:
STOCK Tg(Polr3e-EGFP)DR24Gsat/Mmucd
Stock Number:
010533-UCD
Citation ID:
RRID:MMRRC_010533-UCD
Major Collection:
GENSAT

Strain Information

Gene Details

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26939
Homologene: 14052

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Tg(Polr3e-EGFP)DR24Gsat
Name: transgene insertion DR24, GENSAT Project at Rockefeller University
Synonyms: Tg(Polr3e-EGFP)24Gsat, Founder Line DR24
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
Genotype modified to contain multiple copies of a modified BAC in which EGFP reporter gene is inserted immediately upstream of the coding sequence of the targeted gene. The BAC address for this line is RP23-228B9.
Genotype Determination
MMRRC's Strain Specific PCR
MMRRC's EGFP PCR Design
Donor's EGFP PCR

Strain Description

Phenotype
Site specific expression of green fluorescent protein. BAC-transgenic images of the central nervous system at various ages of the transgenic mouse can be found on the GENSAT project page. In situ histochemistry images using the BGEM probe are also located on the GENSAT website.
MeSH Terms
  • Animals
  • Axons/metabolism
  • Cell Differentiation
  • Cell Lineage
  • Cell Movement
  • Central Nervous System/cytology
  • Central Nervous System/metabolism
  • Chromosomes, Artificial, Bacterial/genetics
  • Cyclic Nucleotide Phosphodiesterases, Type 1
  • Gene Expression Profiling/methods
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Genes, Reporter/genetics
  • Genetic Vectors/genetics
  • Green Fluorescent Proteins
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • LIM-Homeodomain Proteins
  • Luminescent Proteins/analysis
  • Luminescent Proteins/genetics
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Neurosciences/methods
  • Phosphoric Diester Hydrolases/genetics
  • Phosphoric Diester Hydrolases/metabolism
  • Synapses/metabolism
  • Transcription Factors
  • Transgenes/genetics
Strain Development
Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to Swiss Webster mice each generation thereafter.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Research Tools

Strain Origin

Donor
Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT
Primary Reference
Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25. (Medline PMID: 14586460)

Colony and Husbandry Information

Special Considerations

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
White

Breeding

MMRRC Breeding System
Backcross, Sib-matting or Outcross to Swiss Webster
Breeding Scheme(s)
  • Backcross:
    • Wild-type (C57BL/6J) female x Hemizygous Tg male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Hemizygous Tg male littermate or reciprocal mating
  • Outcross:
    • Wild-type (Swiss Webster) female x Hemizygous Tg male littermate or reciprocal mating
Generation
N1F2
Overall Breeding Performance
Undetermined

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Undetermined Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
8
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
010533-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
010533-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
010533-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.