Allele Symbol: Apoa5tm1Hgc
Name: targeted mutation 1, Edward M Rubin
Alteration at locus: Knockout
AV gene was targeted by inserting a neomycin cassette between the Xho1 and EcoRI restriction sites in the AV gene. The neomycin cassette replaces the three exons predicted to encode Apoav.
ES Cell Line: Not specified derived from 129X1/SvJ
Homozygous: Homozygous Apoav knockout mice (-/-)have about four times as much plasma triglyceride as their wild-type littermates. Characterization of lipoprotein particles by fast-protein liquid chromatography (FPLC) and gradient-gel electrophoresis (GGE) reveal that levels of very low density lipoprotein (VLDL) particles are increased in the homozygous knockout mice.
Heterozygous: Heterozygous Apoav knockout mice (+/-)show elevated levels of plasma triglycerides. VLDL levels in a heterozygous knockout mouse were intermediate between the homozygous knockout and control mouse.
Founder genetic background: 129X1/SvJ
Strain genetic background: FVB.129X1(B6)
Strain Development: Targeted clones (129/SvJ) were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6J females. Agouti offspring were tested for germ line transmission of the targeted allele by PCR and heterozygous animals were intercrossed to obtain homozygous deletion.
Suggested Control Mice: Wild-type littermates or FVB mice
Donor: Edward M. Rubin, Ph.D., Lawrence Berkeley National Laboratory
Pennacchio LA, Olivier M, Hubacek JA, Cohen JC, Cox DR, Fruchart JC, Krauss RM, Rubin EM. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science. 2001 Oct 5;294(5540):169-73. (Medline PMID: 11588264 )
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Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
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|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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