Strain Name: C57BL/6-Mbtps1wrt/Mmucd
Stock Number: 012829-UCD
Other Names:
- woodrat, C57BL/6-Mbtps1wrt/Mmcd
Gene Details: (provided by MGI)
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Allele Symbol: Mbtps1wrt Name: woodrat Alteration at locus: Chemically Induced |
Genetic Alterations:
486A->G, Y501C
Genotype Determination:
ES Cell Line: Not applicable
Strain Description
Phenotype
Homozygous phenotype: The mutant mice
show progressive hypopigmentation of the fur (grey), with intra- and
inter-hair variegation. The blood cholesterol level is decreased by 75
percent as compared to wild-type values. The mice are sensitive to mouse
cytomegalovirus (MCMV) infections. The killing function of cytotoxic T
cells (CTL) is impaired.
Heterozygous phenotype: The heterozygous mice appear to be wild-type for the phenotypes tested by donor.
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
Mbtps1wrt/Mbtps1wrt
C57BL/6J-Mbtps1<wrt>- mortality/aging
- increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:146454)
- over 90% of mice die within 30 hours of injection with tunicamycin (an endoplasmic reticulum stressor) compared to under 10% for controls
- increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:146454)
- pigmentation phenotype
- reproductive system phenotype
- reduced female fertility (MGI Ref ID J:123123)
- homozygous mutant females are sterile when bred with homozygous males, and crosses with heterozygous males only give birth to heterozygous pups
- reduced female fertility (MGI Ref ID J:123123)
- homeostasis/metabolism phenotype
- decreased circulating cholesterol level (MGI Ref IDs J:94077, J:123123)
- homozygous mice have significantly reduced levels of serum cholesterol (MGI Ref ID J:123123)
- mice have normal levels of triglycerides (MGI Ref ID J:123123)
- in homozygotes, the blood cholesterol level is decreased by 75 percent as compared to wild-type values (MGI Ref ID J:94077)
- decreased circulating HDL cholesterol level (MGI Ref ID J:123123)
- homozygous mice have significantly reduced levels of HDL
- increased circulating LDL cholesterol level (MGI Ref ID J:123123)
- homozygous mice have elevated levels of LDL
- increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:146454)
- over 90% of mice die within 30 hours of injection with tunicamycin (an endoplasmic reticulum stressor) compared to under 10% for controls
- decreased circulating cholesterol level (MGI Ref IDs J:94077, J:123123)
- immune system phenotype
- decreased susceptibility to bacterial infection (MGI Ref ID J:123123)
- homozygotes are resistant to Listeria infections
- defective cytotoxic T cell cytolysis (MGI Ref ID J:94077)
- in homozygotes, the killing function of cytotoxic T cells (CTL) is impaired
- increased interleukin-1 beta secretion (MGI Ref ID J:146454)
- colon explants from dextran sulfide sodium (DSS) treated mice produce more IL-1beta
- increased interleukin-6 secretion (MGI Ref ID J:146454)
- colon explants produce more IL-6 than controls
- increased susceptibility to induced colitis (MGI Ref ID J:146454)
- mice have an exaggerated colitic response to 2% dextran sulfide sodium (DSS) treatment of drinking water
- starting with 4 days of DSS treatment, mice lose significantly more weight than controls
- by 7 days of DSS treatment, mice have lost almost 20% of their initial weight compared to 10% for controls
- after 9 days of DSS treatment, colons are thickened and shortened with bleeding visible
- histological analysis reveals dramatic leukocyte recruitment and crypt loss after 7 days of DSS treatment
- colon explants from DSS treated mice produce more IL-1beta
- bone marrow chimera experiments indicate non-hematopoietic cells expressing the mutant allele increases susceptibility to induced colitis
- tunicamycin injection causes a colitis-like disease in mutant mice but not in control mice
- increased susceptibility to viral infection (MGI Ref IDs J:94077, J:123123)
- homozygotes are susceptible to MCMV infections (MGI Ref ID J:123123)
- mice present reduced levels of gamma interferon but elevated levels of interleukine 6, 12 and type 1 interferon 36 hours post infection (MGI Ref ID J:123123)
- mice are sensitive to mouse cytomegalovirus (MCMV) infections (MGI Ref ID J:94077)
- decreased susceptibility to bacterial infection (MGI Ref ID J:123123)
- cellular phenotype
- abnormal cell physiology (MGI Ref ID J:123123)
- expression of ATF6-dependent unfolded protein response genes is reduced in homozygous mutants upon activation of the endoplasmic reticulum stress
- abnormal cell physiology (MGI Ref ID J:123123)
- digestive/alimentary phenotype
- increased susceptibility to induced colitis (MGI Ref ID J:146454)
- mice have an exaggerated colitic response to 2% dextran sulfide sodium (DSS) treatment of drinking water
- starting with 4 days of DSS treatment, mice lose significantly more weight than controls
- by 7 days of DSS treatment, mice have lost almost 20% of their initial weight compared to 10% for controls
- after 9 days of DSS treatment, colons are thickened and shortened with bleeding visible
- histological analysis reveals dramatic leukocyte recruitment and crypt loss after 7 days of DSS treatment
- colon explants from DSS treated mice produce more IL-1beta
- bone marrow chimera experiments indicate non-hematopoietic cells expressing the mutant allele increases susceptibility to induced colitis
- tunicamycin injection causes a colitis-like disease in mutant mice but not in control mice
- increased susceptibility to induced colitis (MGI Ref ID J:146454)
- integument phenotype
Founder genetic background: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: The woodrat mutation is recessive. It was first isolated as a homozygous G3 male. This original mouse was bred to C57BL/6 females, and the second generation was backcrossed to the mutant founder. The woodrat homozygous mutant females have low fertility, thus the stock is currently maintained as homozygous male bred with heterozygous females.
Suggested Control Mice:
- Wildtype littermates
Research Applications
- Cardiovascular
- Cell Biology
- Immunology and Inflammation
- Metabolism
- Research Tools
- Virology
Strain Origin
Donor: Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference:
- Brandl K; Rutschmann S; Li X; Du X; Xiao N; Schnabl B; Brenner DA; Beutler B, Enhanced sensitivity to DSS colitis caused by a hypomorphic Mbtps1 mutation disrupting the ATF6-driven unfolded protein response., Proc Natl Acad Sci U S A 2009 Mar 3;106(9):3300-5 (Medline PMID: 19202076)
- For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
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| Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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