Strain Name:
YS/WfLmMmmh
Stock Number:
000138-MU
Citation ID:
RRID:MMRRC_000138-MU
Major Collection:

Strain Information

Avy
Name: nonagouti; agouti viable yellow
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Ednrbs
Name: endothelin receptor type B; piebald
Synonyms: pied spotting, s
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Spontaneous Mutation
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Genetic Alterations
Insertion of an intracisternal A particle element 100 kb upstream of the agouti coding sequences.
Genotype Determination
Phenotype
This inbred strain has at least 2 coat color gene abnormalities. While homozygous for a mutation at the piebald locus (Ednrbs), the mutation of interest is at the A locus. Mice with the target mutation are yellow to agouti in coat color and develop obesity and diabetes associated with the yellow coat color. The target mutation is lethal in the homozygous state. Wild-type mice are black; since the strain is maintained in the heterozygous state, wild-type controls are produced at each generation.
Mammalian Phenotype Terms
Allelic Composition: Avy/Avy (Genetic Background: involves: C3H/HeJ )

Allelic Composition: Avy/Aw (Genetic Background: 129S1.C3-Avy/Aw Chr 19MOLF/Ei )

Allelic Composition: Avy/a (Genetic Background: involves: C3H/HeJ * C57BL/6 )

Allelic Composition: Avy/a (Genetic Background: involves: C57BL/6J )

Allelic Composition: Ednrbs/Ednrbs (Genetic Background: Not Specified )

Allelic Composition: Ednrbs/Ednrbs-13Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-17FrS (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-17Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Acrg (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Chlc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1MLPf (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Pu (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1XMLP (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1XMLPc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-24Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-27Pu (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-27Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-29Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2CHLd (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2Etopc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2MLPl (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-12MNURn (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs-15DttMb/Ednrbs (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-3CHLo (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Chlc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-36Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-31Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-3Etopf (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-48UThc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Blec (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-52Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-9ThW (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbtm1Ywa/Ednrbs (Genetic Background: involves: 129S/SvEv )

MeSH Terms
  • Agouti Signaling Protein
  • Alleles
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Hair Color/genetics
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mice, Obese/genetics
  • Molecular Sequence Data
  • Mutation
  • Proteins/genetics
  • 5-Methylcytosine
  • Betaine/metabolism
  • Choline/metabolism
  • Cytosine/analogs & derivatives
  • Cytosine/metabolism
  • Dietary Supplements
  • Female
  • Folic Acid/metabolism
  • Gene Expression Regulation
  • Genomic Imprinting
  • Male
  • Methionine/metabolism
  • Mice, Inbred Strains
  • Pregnancy
  • S-Adenosylmethionine/metabolism
  • Vitamin B 12/metabolism
  • Zinc/metabolism
  • Disease Susceptibility
  • Embryonic and Fetal Development
  • Hair Color
  • Mice, Mutant Strains
  • Neoplasms, Experimental/genetics
  • Obesity/genetics
  • Phenotype
  • Pigments, Biological/analysis
  • Syndrome
Strain Development
The YS strain was started in 1948 when Chase [Herman B.] introduced recessive spotting into a yellow stock from The Jackson Laboratory and then crossed the stock with C57BL mice (Staats 1972). Breeding stock was supplied by Dr. Chase to GL Wolf at The Institute for Cancer Research; now Fox Chase Cancer Center, Philadelphia, in 1959; the YS/ChWf substrain was maintained by sib-mating since then. (PMID:4405752)

This strain was moved to NCTR in 1972 and maintained by sib-mating with forced heterozygosis by GLW. In 1977, it was derived by caesarian-section and introduced into the SPF barrier colony maintained by NCTR. It was then transferred to ML Lamoreux, who submitted it to the MMRRC.
Suggested Control Mice

Wild-type (black) littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Diabetes and obesity
  • Maternal epigenetics
  • Pigmentation research
Donor
Lynn Lamoreux, Ph.D., Texas Agricultural and Mechanical University.
George L. Wolff, Ph.D., National Center for Toxicological Research, United States Department of Agriculture.
Primary Reference

Duhl DM; Vrieling H; Miller KA; Wolff GL; Barsh GS, Neomorphic agouti mutations in obese yellow mice., Nat Genet 1994 Sep;8(1):59-65. (Medline PMID: 7987393)

Wolff GL. Kodell RL. Moore SR. Cooney CA. Maternal epigenetics and methyl supplements affect agouti gene expression in Avy/a mice. FASEB Journal. 12(11):949-57, 1998 Aug. (Medline PMID: 9707167)

Wolff GL. Roberts DW. Galbraith DB. Prenatal determination of obesity, tumor susceptibility, and coat color pattern in viable yellow (Avy/a) mice. The yellow mouse syndrome. Journal of Heredity. 77(3):151-8, 1986 May-Jun. (Medline PMID: 3734404)

Wolff GL, Pitot HC. Influence of background genome on enzymaticcharacteristics of yellow (A v -, A vy -) mice. Genetics. 1973 Jan;73(1):109-23. (Medline PMID: 4405752)

Colony and Husbandry Information

Pair-breeding is recommended due to large litter sizes. For optimum breeding performance, feed mouse chow that contains 9 percent fat.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Yellow (Heterozygous) / Black (Wildtype)
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous (Yellow) Female x Wildtype (Black) Male
  • Wildtype (Black) Female x Heterozygous (Yellow) Male
Generation
F20+
Overall Breeding Performance
Good. Yellow mice become obese and consequently show a decline in reproduction earlier than black mice. Females with more agouti color have a longer reproductive lifespan compared with yellow females.
Average litter size
Unknown
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000138-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000138-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.