Strain Name:
B6.C3-DctSlt-lt/Mmmh
Stock Number:
000150-MU
Citation ID:
RRID:MMRRC_000150-MU
Major Collection:

Strain Information

DctSlt-lt
Name: dopachrome tautomerase; slaty light
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Dct
Name: dopachrome tautomerase
Synonyms: tyrosinase-related protein-2, TRP-2, Tyrp-2, Tyrp2, TRP2
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Spontaneous Mutation
NCBI: 13190
VEGA: 14
HGNC: HGNC:2709
Homologene: 1447
Genetic Alterations
Point mutation (transition) in gene.
Genotype Determination
Phenotype
Mice homozygous for the mutant allele have a medium gray hair coat. Dopachrome tautomerase is one of the three enzymes that function in the melanogenic pathway.
Mammalian Phenotype Terms
Allelic Composition: DctSlt-lt/DctSlt-lt (Genetic Background: C3H/HeJ )

Allelic Composition: DctSlt-lt/Dct+ (Genetic Background: C3H/HeJ )

MeSH Terms
  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Conserved Sequence
  • DNA Primers
  • Exons
  • Genomic Library
  • Humans
  • Intramolecular Oxidoreductases
  • Introns
  • Isomerases/biosynthesis
  • Isomerases/genetics
  • Mice/genetics
  • Molecular Sequence Data
  • Monophenol Monooxygenase/genetics
  • Multigene Family
  • Point Mutation
  • Polymerase Chain Reaction
  • Regulatory Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid
  • Agouti Signaling Protein
  • Chromatography, High Pressure Liquid
  • Genotype
  • Glycoproteins/genetics
  • Intercellular Signaling Peptides and Proteins
  • Intramolecular Oxidoreductases/genetics
  • Melanins/chemistry
  • Melanins/genetics
  • Melanocytes/metabolism
  • Membrane Glycoproteins
  • Mice
  • Mice, Congenic
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Models, Chemical
  • Mutation
  • Oxidoreductases
  • Phenotype
  • Pigmentation/genetics
  • Proteins/genetics
  • Receptors, Corticotropin/genetics
  • Receptors, Melanocortin
  • Spectrophotometry
Strain Development
Spontaneous mutation occurred on the C3H/HeJ. Backcrossed to C57BL/6J over 10 generations.
Suggested Control Mice
  • C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Coat Color
  • Cell Biology
Donor
Dr. ML Lamoreux, Texas A&M University
Primary Reference
  • Budd PS. Jackson IJ. Structure of the mouse tyrosinase-related protein-2/dopachrome tautomerase (Tyrp2/Dct) gene and sequence of two novel slaty alleles. Genomics. 29(1):35-43, 1995 Sep 1. (Medline PMID: 8530099)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline PMID:11277491)

Colony and Husbandry Information

Maintain on a 9% fat diet. Note wean age.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Medium gray
MMRRC Breeding System
Sib mating, with occassional backcross to C57BL/6.
Breeding Scheme(s)
  • Homozygous mutant female x Homozygous mutant male
  • Homozygous mutant female x Homozygous wildtype male
  • Homozygous wildtype female x Homozygous mutant male
Generation
Unknown
Overall Breeding Performance
Good (similar to C57BL/6)
Average litter size
Unknown
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000150-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000150-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.