Strain Name:
B6.129P2-Rfx5tm1.1Ifo/Mmucd
Stock Number:
016818-UCD
Citation ID:
RRID:MMRRC_016818-UCD
Other Names:
RFX5KO, B6.129P2-Rfx5tm1.1Ifo/Mmcd

Strain Information

Rfx5tm1Ifo
Name: regulatory factor X, 5 (influences HLA class II expression); targeted mutation 1, Irmgard Foerster
Synonyms: Rfx5flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Rfx5
Name: regulatory factor X, 5 (influences HLA class II expression)
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 53970
HGNC: HGNC:9986
Genetic Alterations
Deletion of exon 4-7 through insertion of loxP sites and subsequent Cre-mediated recombination.
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype
Homozygous: Severe reduction in MHC class II expression; impairment of thymic positive selection of CD4 T cells; immunodeficiency

Heterozygous: no phenotype
MeSH Terms
  • Animals
  • B-Lymphocytes/immunology
  • DNA-Binding Proteins/genetics
  • Dendritic Cells/immunology
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Genes, MHC Class II
  • Genetic Vectors
  • H-2 Antigens/biosynthesis
  • H-2 Antigens/genetics
  • Histocompatibility Antigens Class II/biosynthesis
  • Immunologic Deficiency Syndromes/genetics
  • Interferon-gamma/pharmacology
  • Liver/immunology
  • Lymphocyte Activation
  • Macrophages, Peritoneal/drug effects
  • Macrophages, Peritoneal/immunology
  • Mice
  • Mice, Knockout
  • Mutation
  • RNA, Messenger/analysis
  • Regulatory Factor X Transcription Factors
  • Spleen/immunology
  • Thymus Gland/immunology
  • Cell Culture Techniques
  • DNA-Binding Proteins/deficiency
  • Flow Cytometry
  • Histocompatibility Antigens Class II/genetics
  • Immunity
  • Mice, Inbred C57BL
  • Nuclear Proteins/deficiency
  • Nuclear Proteins/genetics
  • T-Lymphocytes, Helper-Inducer/immunology
  • Trans-Activators/deficiency
  • Trans-Activators/genetics
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
Strain Development
The knockout was generated in the E14K cell line and mutant mice were backcrossed to C57BL/6 after germline transmission. After 7 backcross generations mice were intercrossed to obtain homozygous mutant mice.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology
Donor
Irmgard Foerster, Ph.D., Institut fuer umweltmedizinische Forschung
Primary Reference
  • Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Forster I, Reith W. Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8(2):143-55. (Medline PMID: 9491996)
  • Buch T, Polic B, Clausen BE, Weiss S, Akilli-Ozturk O, Chang CH, Flavell R, Schulz A, Jonjic S, Waisman A, Forster I. MHC class II expression through a hitherto unknown pathway supports T helper cell-dependent immune responses: implications for MHC class II deficiency. Blood. 2006 Feb 15;107(4):1434-44. Epub 2005 Oct 27. (Medline PMID: 16254146)

Colony and Husbandry Information

SPF (immunodeficient!)

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016818-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
016818-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
016818-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.