Strain Name:
C57BL/6-Edn3ls/Mmmh
Stock Number:
000181-MU
Citation ID:
RRID:MMRRC_000181-MU
Major Collection:

Strain Information

Edn3ls
Name: endothelin 3; lethal spotting
Synonyms: ls
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Edn3
Name: endothelin 3
Synonyms: 114CH19, 114-CH19, tmgc48
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 13616
HGNC: HGNC:3178
Homologene: 88
Genetic Alterations
Spontaneous Mutation
Genotype Determination
Phenotype
Spotted coat. The heterozygous mouse has an increase in the length of the white tail tip.
Description of White Spotting mutants
Mammalian Phenotype Terms
Allelic Composition: Edn3ls/Edn3ls (Genetic Background: involves: C57BL )

Allelic Composition: Edn3ls/Edn3+ (Genetic Background: involves: C57BL )

MeSH Terms
  • Animals
  • Melanocytes/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Phenotype
  • Skin Pigmentation/genetics
  • Base Sequence
  • Chromosome Mapping
  • Crosses, Genetic
  • Disease Models, Animal
  • Genes, Lethal
  • Genetic Linkage
  • Hair Color/genetics
  • Haplotypes/genetics
  • Hirschsprung Disease/embryology
  • Hirschsprung Disease/genetics
  • Humans
  • Mice, Mutant Strains/genetics
  • Molecular Sequence Data
  • Muridae/genetics
  • Neural Crest/pathology
  • Species Specificity
  • Amino Acid Sequence
  • Endothelins/deficiency
  • Endothelins/genetics
  • Endothelins/physiology
  • Female
  • Homozygote
  • Male
  • Mice, Knockout
  • Mice, Mutant Strains
  • Point Mutation
  • Receptor, Endothelin B
  • Receptors, Endothelin/physiology
  • Embryonic and Fetal Development
  • Endothelin-3/genetics
  • Gene Expression Regulation, Developmental
  • Mice, Transgenic
  • Nervous System/embryology
  • Piebaldism/genetics
  • Receptors, Endothelin/genetics
  • Cell Differentiation
  • Endothelin-3/metabolism
  • Endothelins/metabolism
  • Gene Expression Regulation
  • Hirschsprung Disease/metabolism
  • Melanocytes/metabolism
  • Models, Biological
  • Mutation
  • Neural Crest/metabolism
  • Neurons/metabolism
  • RNA, Messenger/metabolism
  • Receptors, Endothelin/metabolism
  • Signal Transduction
Strain Development
Mutant backcrossed to C57BL/6J to eliminate at and possible confounding effects in pigmentation studies involving Edn3ls. Donating Investigator created congenic B6 mice and JU mice that were lethal-spotted and were non-agouti (as is normal for both B6 and JU).
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigment cell research
  • Development
  • Hirschsprung Disease. Note: Endothelin3 (encoded at lethal spotting locus) must interact with Ednrb (encoded at piebald locus) if Hirschsprung's disease is to be prevented.
Donor
Lynn Lamoreux, Ph.D., Texas A&M University
Primary Reference
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Lamoreux, ML. Pigment cell research 12(6):383-90, 1999. (Medline PMID: 10614578)
  • Pavan WJ, Liddell RA, Wright A, Thibaudeau G, Matteson PG, McHugh KM, Siracusa LD. A high-resolution linkage map of the lethal spotting locus: a mouse model for Hirschsprung disease. Mamm Genome. 1995 Jan;6(1):1-7. (Medline PMID: 7719019)
  • Baynash AG, Hosoda K, Giaid A, Richardson JA, Emoto N, Hammer RE, Yanagisawa M. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell. 1994 Dec 30;79(7):1277-85. (Medline PMID: 8001160)
  • Rice J, Doggett B, Sweetser DA, Yanagisawa H, Yanagisawa M, Kapur RP. Transgenic rescue of aganglionosis and piebaldism in lethal spotted mice. Dev Dyn. 2000 Jan;217(1):120-32. (Medline PMID: 10679935)
  • McCallion AS, Chakravarti A. EDNRB/EDN3 and Hirschsprung disease type II. Pigment Cell Res. 2001 Jun;14(3):161-9. (Medline PMID: 11434563)

Colony and Husbandry Information

Feed 9% fat breeder chow.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
homozygous- white spotted
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Mate heterozygous mice to heterozygous littermates or backcross to C57BL/6J mice.
Generation
N>10
Overall Breeding Performance
Good. Heterozygous mice can be difficult to distiguish from wild type mice. If backcross mating is performed, it is best to breed suspected heterozygous mice to suspected heterozygous females to confirm that breeders are indeed heterozygous through the production of spotted offspring prior to the backcross mating. Breeder pairs should be confirmed heterozygous by looking for the presence of spotted homozygous pups.
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000181-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000181-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000181-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.