Strain Name:
JU.Cg-Ay/a/Mmmh
Stock Number:
000221-MU
Citation ID:
RRID:MMRRC_000221-MU
Major Collection:

Strain Information

Ay
Name: nonagouti; agouti yellow
Synonyms: Ay, A(y)
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Genetic Alterations
The mutation appears to be a DNA structural alteration that disrupts a gene, hnRNP associated with lethal yellow (Raly), 5' to the agouti locus and places the agouti locus under the control of the Raly promotor.
Genotype Determination
Phenotype
The mutant allele is characterized by yellow color, obesity, and diabetes in heterozygotes. It is prenatal lethal in the homozygous state. Therefore, black offspring will be produced every generation. In mice mutant at the agouti locus, these phenotypes are all reduced if the phenotype of the mouse is more eumelanic than pheomelanic,as is the case when the same mutant allele is backcrossed onto the JU/CtLm (MMRRC:000136) inbred background. The mice are yellow until the first molt, then become umbrous (darker dorsally). After about 6 months of age, this umbrous phenotype is gradually lost.
Mammalian Phenotype Terms
Allelic Composition: Ay/Ay (Genetic Background: 101-Ay )

  • mortality/aging
  • other phenotype
Allelic Composition: Ay/Aw (Genetic Background: 129S1.C3-Ay/Aw Chr 19MOLF/Ei )

Allelic Composition: Ay/a (Genetic Background: B6.Cg-Ay/J )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay/a (Genetic Background: involves: KK )

MeSH Terms
  • Agouti Signaling Protein
  • Alleles
  • Animals
  • Chromosome Mapping
  • Diabetes Mellitus, Type 2/genetics
  • Genes, Dominant/genetics
  • Genes, Lethal/genetics
  • Genes, Recessive/genetics
  • Hair Color/genetics
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mice, Mutant Strains/genetics
  • Muridae/genetics
  • Mutation/genetics
  • Neoplasms, Experimental/genetics
  • Obesity/genetics
  • Proteins/genetics
  • Recombination, Genetic/genetics
  • Sequence Deletion
  • Chromatography, High Pressure Liquid
  • Genotype
  • Glycoproteins/genetics
  • Intramolecular Oxidoreductases/genetics
  • Melanins/chemistry
  • Melanins/genetics
  • Melanocytes/metabolism
  • Membrane Glycoproteins
  • Mice, Congenic
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Models, Chemical
  • Monophenol Monooxygenase/genetics
  • Mutation
  • Oxidoreductases
  • Phenotype
  • Pigmentation/genetics
  • Receptors, Corticotropin/genetics
  • Receptors, Melanocortin
  • Spectrophotometry
  • Agouti-Related Protein
  • Epitopes/metabolism
  • Melanophores/cytology
  • Melanophores/drug effects
  • Melanophores/metabolism
  • Pigmentation/drug effects
  • Pigmentation/physiology
  • Protein Binding
  • Proteins/metabolism
  • Proteins/pharmacology
  • Receptors, Corticotropin/metabolism
  • Receptors, Corticotropin/physiology
  • Recombinant Proteins/genetics
  • Xenopus
  • alpha-MSH/metabolism
  • alpha-MSH/pharmacology
Strain Development
Spontaneous mutation maintained by backcross to wildtype JU/CtLm (MMRRC:000136) mice.
Suggested Control Mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigmentation
  • Diabetes/Obesity
  • Reproduction
Donor
Dr. M. L. Lamoreux, Texas A&M University
Primary Reference
  • Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline PMID: 8146154)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline PMID: 11277491)
  • Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline PMID: 9450927)

Colony and Husbandry Information

Feed mouse chow that contains 4 percent fat.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Yellow
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • JU/CtLm Wildtype Female x Heterozygous Yellow Male
  • Heterozygous Yellow Female x JU/CtLm Wildtype Male
  • Heterozygous Yellow Female x Heterozygous Yellow Male
Generation
N10+
Overall Breeding Performance
Good for young mice but quickly drops off as mice age. Both males and females quickly become sterile if they become obese.
Average litter size
Unknown
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000221-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000221-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000221-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.