Allele Symbol: Ay Name: agouti yellow Chromosome: 2 Alteration at locus: Spontaneous Mutation
The mutation appears
to be a DNA structural alteration that disrupts a gene, hnRNP
associated with lethal yellow (Raly), 5' to the agouti locus and
places the agouti locus under the control of the Raly promotor.
Phenotype The mutant allele is
characterized by yellow color, obesity, and diabetes in heterozygotes. It is
prenatal lethal in the homozygous state. Therefore, black
offspring will be produced every generation. In mice mutant at the
agouti locus, these phenotypes are all reduced if the phenotype of
the mouse is more eumelanic than pheomelanic,as is the case when
the same mutant allele is backcrossed onto the JU/CtLm (MMRRC:000136) inbred background. The
mice are yellow until the first molt, then become umbrous (darker
dorsally). After about 6 months of age, this umbrous phenotype is
Mammalian Phenotype Terms:(provided by MGI)Extend all MPTs assigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
adipose tissue phenotype
increased brown adipose tissue amount (MGI Ref ID J:131039)
female mice have a 6-fold gain in weight of BAT compared to littermate controls
increased gonadal fat pad weight (MGI Ref ID J:131039)
female mice have almost a 7-fold gain in weight of fat pad
increased inguinal fat pad weight (MGI Ref ID J:131039)
female mice have a greater than 6-fold gain in weight of fat pad
Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ,
Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse
lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6.
Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and
pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31.
Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes
Dev. 1998 Feb 1;12(3):316-30.
Colony and Husbandry Information
Coat color: Yellow
MMRRC Breeding System: Backcross
JU/CtLm Wildtype Female x Heterozygous Yellow Male
Heterozygous Yellow Female x JU/CtLm Wildtype Male
Heterozygous Yellow Female x Heterozygous Yellow Male
Overall Breeding Performance: Good for young mice but quickly drops off as mice age. Both males and females quickly become sterile if they become obese.
Viability and Fertility:
Homozygotes are viable:
Homozygotes are fertile:
Heterozygotes are fertile:
Age Reproductive Decline:
Average litter size: Unknown
Recommended wean age: 4 weeks
Feed mouse chow that contains 4 percent fat.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Order Request Information
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU).
A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded
to your institutional official for completion.
Additional charges may apply for any special requests. Shipping
costs are in addition to the basic distribution/resuscitation
fees. Information on shipping costs and any additional charges will be provided
by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #
Fee/unit (US $)
Litter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Recovered litter1; additional fees for any special requests.
$437.00 / $817.00
Non-Profit / For-Profit
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Approximate quantity3: 20-40 embryos / aliquot
The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
An aliquot contains a sufficient number of embryos (in one or more
vials and based on the transfer success rate of the MMRRC facility) to
transfer to at least two recipients. The MMRRC makes no guarantee concerning
embryo transfer success experienced in the recipient investigator's laboratory.
Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the