Allele Symbol: Mir155tm1.1Brd
Name: targeted mutation 1.1, Allan Bradley
Alteration at locus: Knockout
Allele Symbol: Tyrc-Brd
Name: albino, Allan Bradley
Alteration at locus: Knockout
A small deletion was created to remove exon 2 of the Bic/miR155 locus. This is a null allele for the locus.
ES Cell Line: AB2.2
Homozygous phenotype: Immuno-compromised mice. Defective responses by B/T cells and dendritic cells.
Heterozygous phenotype: None
Strain genetic background: N5 mixed B6/129S7
Strain Development: Original allele targeted on AB2.2 ES cells. Males chimaeras were mated directly to C57BL/6 females. The original allele contained a floxed NEO cassette. To remove the NEO cassette the mice were bred to CMV-Cre deleter strain. Mice were then backcrossed 5 generations onto C57BL/6 genetic background (see publication for details).
Suggested Control Mice:
Donor: Allan Bradley, Ph.D., Wellcome Trust Sanger Institute
Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P, Soond DR, van Dongen S, Grocock RJ, Das PP, Miska EA, Vetrie D, Okkenhaug K, Enright AJ, Dougan G, Turner M, Bradley A. Requirement of bic/microRNA-155 for normal immune function. Science. 2007 Apr 27;316(5824):608-11. (Medline PMID: 17463290)
The mice are immune-deficient. Helicobacter minus facility will be required to avoid spontaneous inflammatory bowel disease in this strain.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email email@example.com.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH