Strain Name:
B6.129X1-Dmbt1tm1Kumc/Mmmh
Stock Number:
030289-MU
Citation ID:
RRID:MMRRC_030289-MU

Strain Information

Dmbt1tm1Kumc
Name: deleted in malignant brain tumors 1; targeted mutation 1, Robert C De Lisle
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Genetic Alterations
Neo and GFP cassettes replace exon 1 and part of intron 1. The GFP cassette is non- functional (no mRNA or GFP protein can be detected).
Genotype Determination
ES Cell Line
RW-4 derived from 129X1/SvJ
Phenotype
Homozygous: Slowed protein traffic in the regulated secretory pathway of the exocrine pancreatic acinar cell.

Heterozygous: Same as wild-type
Mammalian Phenotype Terms
Allelic Composition: Dmbt1tm1Kumc/Dmbt1tm1Kumc (Genetic Background: B6.129X1-Dmbt1tm1Kumc )

Allelic Composition: Dmbt1tm1Kumc/Dmbt1tm1Kumc (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Amylases/metabolism
  • Animals
  • Blotting, Western
  • Ceruletide
  • Colitis/chemically induced
  • Colitis/genetics
  • Colitis/pathology
  • Dextran Sulfate
  • Electrophoresis, Polyacrylamide Gel
  • Gastrointestinal Neoplasms/epidemiology
  • Gastrointestinal Neoplasms/genetics
  • Gastrointestinal Neoplasms/pathology
  • Gastrointestinal Tract/physiology
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mucins/deficiency
  • Mucins/genetics
  • Pancreas/cytology
  • Pancreas/drug effects
  • Pancreas/secretion
  • Pancreatitis/chemically induced
  • Pancreatitis/pathology
  • Tumor Suppressor Protein p53/deficiency
  • Tumor Suppressor Protein p53/genetics
  • Tumor Suppressor Protein p53/physiology
Strain Development
Targeted mice were backcrossed 8 generations on the C57BL/6J background. They are maintained by breeding homozygous null mice.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Immunology and Inflammation
Donor
Robert C De Lisle, Ph.D., University of Kansas Medical Center
Primary Reference
De Lisle RC, Xu W, Roe BA, Ziemer D. Effects of Muclin (Dmbt1) Deficiency on the Gastrointestinal System. Am J Physiol Gastrointest Liver Physiol. 2008 Mar;294(3):G717-27. Epub 2008 Jan 17. (Medline PMID: 18202109)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030289-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.