Strain Name: C57BL/6J-Tg(Cd4-Il7r)1Wjl/Mmnc
Stock Number: 031041-UNC
Other Names:
Gene Details: (provided by MGI)
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Transgene: Tg(Cd4-Il7r)1Wjl Name: transgene insertion 1, Warren J Leonard Alteration at locus: Transgenic |
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Promoter: Cd4 Name: CD4 antigen Chromosome: 6 Alteration at locus: Transgenic |
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Transgene: Il7r Name: interleukin 7 receptor Chromosome: 15 Alteration at locus: Transgenic |
Genetic Alterations:
To create IL-7R
{alpha} transgenic mice, murine IL-7R{alpha} cDNA was amplified from
mouse splenic T cells by RT-PCR, and the sequence was verified after
cloning into pCR2.1 vector by TA cloning (Invitrogen). The cDNA was then
subcloned into a CD4 enhancer/promoter transgenic vector, which lacks the
CD4 silencer located in intron 1 and thus directs transgene expression in
T lineage cells . The plasmid harboring the IL-7R{alpha} cDNA was then
linearized and injected into C57BL/6 oocytes. The progenies were screened
for the presence of the transgene by Southern blotting. Two positive mice
were used as founders and bred on the C57BL/6 background.
Genotype Determination:
- Genotyping Protocol(s)
- Center protocol and contact for technical support will be shipped with mice.
ES Cell Line: Not applicable
Strain Description
Phenotype
Homozygous phenotype: None
Hemizygous phenotype: None
Founder genetic background: C57BL/6J
Strain genetic background: C57BL/6J
Suggested Control Mice:
- Wildtype littermates
Research Applications
- Immunology and Inflammation
Strain Origin
Donor: Warren Leonard, Ph.D., NIH/NHLBI
Primary Reference:
Haring JS, Jing X, Bollenbacher-Reilley J, Xue HH, Leonard WJ, Harty JT. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008 Mar 1;180(5):2855-62. (Medline PMID: 18292507)
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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