Strain Name:
B6.129S1-Syngap1tm1.2Mabk/Mmucd
Stock Number:
031831-UCD
Citation ID:
RRID:MMRRC_031831-UCD
Other Names:
SynGAP floxxed, B6.129S1-Syngap1tm1.2Mabk/Mmcd

Strain Information

Syngap1tm1.2Mabk
Name: synaptic Ras GTPase activating protein 1 homolog (rat); targeted mutation 1.2, Mary B Kennedy
Synonyms: synGAP flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Targeted Mutation
Syngap1
Name: synaptic Ras GTPase activating protein 1 homolog (rat)
Synonyms: Syngap
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Targeted Mutation
NCBI: 240057
Homologene: 84739
Genetic Alterations
LoxP sites were inserted flanking exons 4-9
Genotype Determination
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: No noticable phenotype of floxed mutants. Homozygous full knockouts die 2-3 days after birth.

Heterozygous: No noticable phenotype of floxed heterozygotes. Normal synaptic plasticity is disrupted in full knockout heterozygotes, producing a variety of learning deficits and morphological alterations in spine synapses.
MeSH Terms
  • Age Factors
  • Animals
  • Animals, Newborn
  • Apoptosis/physiology
  • Brain/growth & development
  • Brain/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neurons/physiology
  • ras GTPase-Activating Proteins/genetics
  • ras GTPase-Activating Proteins/physiology
  • Cells, Cultured
  • Dendritic Spines/chemistry
  • Dendritic Spines/ultrastructure
  • Disks Large Homolog 4 Protein
  • Excitatory Postsynaptic Potentials
  • Guanylate Kinases
  • Hippocampus/cytology
  • Hippocampus/physiology
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins/analysis
  • Neurons/cytology
  • Phenotype
  • Protein Structure, Tertiary
  • ras GTPase-Activating Proteins/chemistry
  • Actin Depolymerizing Factors/metabolism
  • Blotting, Western
  • Brain/metabolism
  • Brain/ultrastructure
  • Cytoskeleton
  • Dendritic Spines/drug effects
  • Dendritic Spines/metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Excitatory Amino Acid Agonists/pharmacology
  • Excitatory Postsynaptic Potentials/drug effects
  • Excitatory Postsynaptic Potentials/physiology
  • Long-Term Synaptic Depression/drug effects
  • Long-Term Synaptic Depression/physiology
  • Microscopy, Confocal
  • N-Methylaspartate/pharmacology
  • Organ Culture Techniques
  • Patch-Clamp Techniques
  • Phosphorylation
  • Receptors, N-Methyl-D-Aspartate/metabolism
  • rac GTP-Binding Proteins/metabolism
  • ras GTPase-Activating Proteins/metabolism
  • ras Proteins/metabolism
Strain Development
The original mouse was outcrossed into C57BL/6 (7 outcrosses)and then the colony has been maintained by breeding mice homozygous for the mutation.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
Donor
Mary B. Kennedy, Ph.D., California Institute of Technology
Primary Reference
  • Knuesel I, Elliott A, Chen HJ, Mansuy IM, Kennedy MB. A role for synGAP in regulating neuronal apoptosis. Eur J Neurosci. 2005 Feb;21(3):611-21. (Medline PMID: 15733080)
  • Vazquez LE, Chen HJ, Sokolova I, Knuesel I, Kennedy MB. SynGAP regulates spine formation. J Neurosci. 2004 Oct 6;24(40):8862-72. (Medline PMID: 15470153)
  • Carlisle HJ, Manzerra P, Marcora E, Kennedy MB. SynGAP regulates steady-state and activity-dependent phosphorylation of cofilin. J Neurosci. 2008 Dec 10;28(50):13673-83. (Medline PMID: 19074040)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031831-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031831-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
031831-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.