Strain Name:
B6.129-Hpgdtm1Bhk/Mmjax
Stock Number:
032028-JAX
Citation ID:
RRID:MMRRC_032028-JAX

Strain Information

Hpgdtm1Bhk
Name: hydroxyprostaglandin dehydrogenase 15 (NAD); targeted mutation 1, Beverly H Koller
Synonyms: Pgdh-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Hpgd
Name: hydroxyprostaglandin dehydrogenase 15 (NAD)
Synonyms: 15-PGDH
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 15446
HGNC: HGNC:5154
Homologene: 68095
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: The targeted mutation deletes the region of the mouse Hpgd locus corresponding to amino acids 167-266 of the human gene. Homozygous (Pgdh-/-) mice exhibit failure of the ductus arteriosus to close; resulting in perinatal lethality within 12-48 hours after birth due to congestive heart failure. Homozygotes are viable only if newborn pups are given indomethacin (6mg/kg) within 12 hours of birth (and these surviving homozygous males are fertile, but surviving homozygous females are infertile). These mice may be useful in studying the role of Hpgd and prostaglandin E2 (PGE2) in closure of the ductus arteriosus and clinical management of ductus-dependant congenital heart defects.

Heterozygous: Heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Hpgdtm1Bhk/Hpgdtm1Bhk (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Hpgdtm1Bhk/Hpgdtm1Bhk (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

MeSH Terms
  • Cyclooxygenase 2
  • Dinoprostone/metabolism
  • Ductus Arteriosus/enzymology
  • Ductus Arteriosus/physiology
  • Female
  • Humans
  • Hydroxyprostaglandin Dehydrogenases/metabolism
  • Infant, Newborn
  • Isoenzymes/metabolism
  • Membrane Proteins
  • Placenta/physiology
  • Pregnancy
  • Prostaglandin-Endoperoxide Synthases/metabolism
  • Pulmonary Circulation
Strain Development
A targeting vector was designed to replace a portion of the mouse Hpgd locus (corresponding to amino acids 167-266 in human 15-hydroxyprostaglandin dehydrogenase (15-PGDH)) with a neomycin-resistance cassette (PGK-Neo). This construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J to establish the colony. These mutant mice were backcrossed at least 15 generations to C57BL/6 mice prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Jax Stock No. 000664) for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Beverly Koller, Ph.D., University of North Carolina at Chapel Hill
Primary Reference
Coggins KG; Latour A; Nguyen MS; Audoly L; Coffman TM; Koller BH, Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus., Nat Med 2002 Feb;8(2):91-2 (Medline PMID: 11821873)

Colony and Husbandry Information

When maintained as a live colony, heterozygous mice are bred with wildtype siblings or C57BL/6J inbred mice (Jax Stock No. 000664). Homozygous mice exhibit perinatal lethality within 12-48 hours after birth due to congestive heart failure. Homozygotes are viable only if newborn pups are given indomethacin (6mg/kg) within 12 hours of birth (and these surviving homozygous males are fertile, but surviving homozygous females are infertile).

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032028-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032028-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.