Strain Detail Sheet

Strain Name: B6.129S6-Ptpn11^tm1Toa/Mmjax

Stock Number: 032103-JAX

Other Names:

Gene Details: (provided by MGI)

Allele Symbol: Ptpn11^tm1Toa
Name: targeted mutation 1, Toshiyuki Araki
Alteration at locus: Targeted Mutation

Genetic Alterations:
A point mutation of D61Y and a restriction enzyme site was inserted into exon3. By homologous recombination & subsequent removal of the neo cassette that was present & flanked by LoxP site in exon 3.

Genotype Determination:

Ptpn11 (exon 3), Sanger sequencing

ES Cell Line: J1 derived from B6.129S6

Strain Description

Phenotype
Homozygous phenotype: Mice that are homozygous for the targeted mutation are not viable.

Heterozygous phenotype: hen used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).

Founder genetic background: 129S6/SvEvTac

Strain genetic background: B6.129S6

Strain Development: A targeting vector was designed to place a loxP-neo-loxP-STOP-loxP cassette between exons 2 and 3 and to insert a D61Y point mutation and unique AgeI restriction enzyme site in exon 3 of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for a minimum of 10 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

Developmental Biology
Hematology
Models for Human Disease

Strain Origin

Donor: Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute

Primary Reference:

Chan G, Kalaitzidis D, Usenko T, Kutok JL, Yang W, Mohi MG, Neel BG. Leukemogenic Ptpn11 causes fatal myeloproliferative disorder via cell-autonomous effects on multiple stages of hematopoiesis. Blood. 2009 Apr 30;113(18):4414-24. Epub 2009 Jan 29. (Medline PMID: 19179468)

Special Considerations

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level:

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution:

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees:

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description

Distribution
Fee/unit (US $)

Units

Notes

032103-JAX-RESUS

Litter recovered from cryo-archive

$2,022.00

Non-Profit

Litter

Recovered litter¹; additional fees for any special requests.

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.