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Availability & Fees Order this Strain
Genotyping Protocol
All b2m-, TAP-1-, and b2m-TAP-1- mice were B6-backcrossed. C57BL6NCr (designated B6 below) mice were bred in the animal facility of the University of California (Berkeley, CA). B6 b2m--deficient (b2m-) mice were established by backcrossing (129/Sv x B6) F1b2m+/- five times to B6 mice, and were maintained by intercrossing 129/Ola TAP-1- mice backcrossed to FVB/N were kindly provided by Drs. M. van Roon and A. Berns (Netherlands Cancer Institute, Amsterdam, The Netherlands), and then were backcrossed five or six times to B6 by Dr. K. Hogquist (Minneapolis, MN).
Wild-type littermates
Dorfman JR, Zerrahn J, Coles MC, Raulet DH. The basis for self-tolerance of natural killer cells in beta2-microglobulin- and TAP-1- mice. J Immunol. 1997 Dec 1;159(11):5219-25. (Medline PMID: 9548460)
Huh GS; Boulanger LM; Du H; Riquelme PA; Brotz TM; Shatz CJ, Functional requirement for class I MHC in CNS development and plasticity, Science 2000 Dec 15;290(5499):2155-9 (Medline PMID: 11118151)
Zijlstra M, Li E, Sajjadi F, Subramani S, Jaenisch R. Germ-line transmissionof a disrupted beta 2-microglobulin gene produced by homologous recombination in embryonic stem cells. Nature. 1989 Nov 23;342(6248):435-8. (Medline PMID: 2685607)
Tourne S, van Santen HM, van Roon M, Berns A, Benoist C, Mathis D, Ploegh H.Biosynthesis of major histocompatibility complex molecules and generation of Tcells in Ii TAP1 double-mutant mice. Proc Natl Acad Sci U S A. 1996 Feb20;93(4):1464-9. (Medline PMID: 8643655)
Colony Surveillance Program and Current Health Reports
Other
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.