Strain Detail Sheet

Strain Name: B6;129S5-Tspan12^tm1Lex/Mmucd

Stock Number: 032693-UCD

Other Names:

Tspan12, UNQ774, B6;129S5-Tspan12^tm1Lex/Mmcd

Gene Details: (provided by MGI)

Allele Symbol: Tspan12^tm1Lex
Name: targeted mutation 1, Lexicon Pharmaceuticals
Alteration at locus: Knockout

Genetic Alterations:
The Tspan12 gene consists of 9 exons. Coding exon 1 was targeted (NCBI accession NM_173007.1) by homologous recombination.

Genotype Determination:

Donor's PCR

ES Cell Line: Lexicon 129/SvEvBrd

Strain Description

Phenotype
Phenotypic analysis of the F2 mice indicates this mutation affects cardiology and opthalmology.

Phenotypic data provided by Lexicon Genetics, Inc.

Founder genetic background: B6;129S5

Strain genetic background: B6;129S5

Strain Development: Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice.

Mice are archived at the sib-mated generation F2. Recovery will be performed using C57BL/6J donors unless otherwise requested.

Suggested Control Mice:

Wild-type littermates

Research Applications

Cancer
Cardiovascular
Familial exudative vitreoretinopathy (FEVR)
Hepatotoxicity
Models for human disease
Research tools
Sensorineural

Strain Origin

Donor: Genentech, Inc.

Primary Reference:

Tang T, Li L, Tang J, Li Y, Lin W, Martin F, Deanna Grant D, Solloway M, Parker L, Weilan Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ. A mouse knockout library for secreted and transmembrane proteins. Nat Biotechnol. 2010 July; 28(7):749–755. (Medline PMID: 20562862)
Junge HJ, Yang S, Burton JB, Paes K, Shu X, French DM, Costa M, Rice DS and Ye W. "TSPAN12 regulates retinal vascular development by promoting Norrin- but not Wnt-induced FZD4/beta-catenin signaling." Cell. 2009 Oct 16;139(2):299-311 (Medline PMID: 19837033).

Colony and Husbandry Information

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

Wild-type female x Heterozygous male
Heterozygous female x Heterozygous male
Homozygous female x Homozygous male

Generation: F2

Overall Breeding Performance: Poor

Reproductive Statistics

Viability and Fertility:	Female	Male
Homozygotes are viable:	Yes	Yes
Homozygotes are fertile:	Yes	Yes
Heterozygotes are fertile:	Yes	Yes

Age Reproductive Decline:	Unknown	Unknown

Average litter size:

Recommended wean age: 3 weeks

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level:

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution:

Distribution of this strain requires execution of the Genentech Lexicon agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Fees:

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description

Distribution
Fee/unit (US $)

Units

Notes

032693-UCD-RESUS

Litter recovered from cryo-archive

$2,022.00

Non-Profit

Litter

Recovered litter¹; additional fees for any special requests.

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.