Strain Name: B6.C(129)-Traf4m1Pav/Mmmh
Stock Number: 033049-MU
Other Names:
Gene Details: (provided by MGI)
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Allele Symbol: Traf4m1Pav Name: mutation 1, William J Pavan Alteration at locus: Chemically Induced |
Genetic Alterations:
C to T mutation
in the 5' splice site of intron 2 that results in skipping of exon 2. The donor has
detected mRNA transcript and predicts it would produce truncated or altered
protein.
Genotype Determination:
ES Cell Line: Not Applicable
Strain Description
Phenotype
Homozygous phenotype: Incompletely
penetrant homozygous lethality. E11.5 embryos have ectopic clusters of
cells at lower dorsal neural tube. Liveborn are runted, sickly, with
craniofacial and eye dysmorphology and hydrocephaly. Liveborn dead by 4
weeks.
Heterozygous phenotype: No
mutant phenotype observed. Normal growth and reproduction.
Strain genetic background: C57BL/6, BALB/c mix
Strain Development: BALB/c male was ENU mutagenized and mated to C57BL/6 Sox10-lacz/+ female. Embryos showed lacz+ ectopic cell clusters at E11.5 at lower dorsal neural tube. ENU mutation was positionally cloned and identified. Heterozygote carriers were mated to C57BL/6J wildtype or C57BL-Sox10-lacz mice, for 5-10 generations.
Suggested Control Mice:
- Wildtype littermates
Research Applications
- Developmental Biology
Strain Origin
Donor: Bill Pavan, Ph.D., NHGRI/NIH
Primary Reference:
Buac K; Watkins-Chow DE; Loftus SK; Larson DM; Incao A; Gibney G; Pavan WJ, A Sox10 expression screen identifies an amino acid essential for Erbb3 function., PLoS Genet 2008 Sep;4(9):e1000177 (Medline PMID: 18773073)
Special Considerations
Liveborn homozygotes need to be weaned late and then weaned to mush because they are runted and sickly. Normal mouse colony husbandry for heterozygotes.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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